ArticleBioTechniques2013
Counting unstained, confluent cells by modified bright-field microscopy.
Article in BioTechniques, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Neuronal safety of Kv11.1 blockers: Patch-clamp and in vitro evaluation with physiologically based pharmacokinetic (PBPK) modeling.Toxicology reports · 2026Article
- High-Throughput Label-Free Continuous Quantification of Muscle Stem Cell Proliferation and Myogenic Differentiation.Stem cell reviews and reports · 2025Article
- Making sense of blobs, whorls, and shades: methods for label-free, inverse imaging in bright-field optical microscopy.Biophysical reviews · 2025Review
- Automated cell counting for Trypan blue-stained cell cultures using machine learning.PloS one · 2023Article
- Automated Raman Micro-Spectroscopy of Epithelial Cell Nuclei for High-Throughput Classification.Cancers · 2021Article
- Engineering Microneedle Patches for Improved Penetration: Analysis, Skin Models and Factors Affecting Needle Insertion.Nano-micro letters · 2021Review
- Disposable Polymeric Nanostructured Plasmonic Biosensors for Cell Culture Adhesion Monitoring.Frontiers in bioengineering and biotechnology · 2021Article
- Integrated use of LC/MS/MS and LC/Q-TOF/MS targeted metabolomics with automated label-free microscopy for quantification of purine metabolites in cultured mammalian cells.Purinergic signalling · 2019Article
- Iterative unsupervised domain adaptation for generalized cell detection from brightfield z-stacks.BMC bioinformatics · 2019Article
- Article
- AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis.Cancer letters · 2017Article
- Robust microbial cell segmentation by optical-phase thresholding with minimal processing requirements.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2017Article
- Acetyl-CoA carboxylase rewires cancer metabolism to allow cancer cells to survive inhibition of the Warburg effect by cetuximab.Cancer letters · 2017Article
- A feed-forward regulation of endothelin receptors by c-Jun in human non-pigmented ciliary epithelial cells and retinal ganglion cells.PloS one · 2017Article
- Identification and validation of COX-2 as a co-target for overcoming cetuximab resistance in colorectal cancer cells.Oncotarget · 2016Article
- Quantification of Calcified Particles in Human Valve Tissue Reveals Asymmetry of Calcific Aortic Valve Disease Development.Frontiers in cardiovascular medicine · 2016Article
- Non-invasive and non-destructive measurements of confluence in cultured adherent cell lines.MethodsX · 2015Article
- Semi-quantitative monitoring of confluence of adherent mesenchymal stromal cells on calcium-phosphate granules by using widefield microscopy images.Journal of materials science. Materials in medicine · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We present a very simple procedure yielding high-contrast images of adherent, confluent cells such as human neuroblastoma (SH-EP) cells by ordinary bright-field microscopy. Cells are illuminated through a color filter and a pinhole aperture placed between the condenser and the cell culture surface. Refraction by each cell body generates a sharp, bright spot when the image is defocused. The technique allows robust, automatic cell counting from a single bright-field image in a wide range of focal positions using free, readily available image-analysis tools. Contrast may be enhanced by swelling cell bodies with a brief incubation in PBS. The procedure was benchmarked against manual and automated counting of fluorescently labeled cell nuclei. Counts from day-old and freshly seeded plates were compared in a range of densities, from sparse to densely overgrown. On average, bright-field images produced the same counts as fluorescence images, with less than 5% error. This method will allow routine cell counting using a plain bright-field microscope without cell-line modification or cell staining.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.