ArticleG3 (Bethesda, Md.)2013
The development of a monoclonal antibody recognizing the Drosophila melanogaster phosphorylated histone H2A variant (γ-H2AV).
Article in G3 (Bethesda, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 89 citations in OpenAlex.
- Dual roles for a chromatin insulator-H2Av axis in the DNA damage response and hematopoietic development in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Dual contributions of Xrp1 to genome integrity through the DNA damage response and cell competition.PLoS genetics · 2026Article
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- Noc1 downregulation induces nucleolar stress and upregulates p53 isoforms, with a robust increase of the truncated p53E isoform in Drosophila wing discs.G3 (Bethesda, Md.) · 2026Article
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- The Drosophila mauritiana synaptonemal complex protein C(3)G repatterns the recombination landscape of Drosophila melanogaster.PLoS genetics · 2025Article
- Abundant piRNA production mediated by theProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Tau reduction impairs nephrocyte function in Drosophila.BMB reports · 2025Article
- Chronic Jet Lag Disrupts Circadian Rhythms and Induces Hyperproliferation in Murine Lacrimal Glands via ROS Accumulation.Investigative ophthalmology & visual science · 2025Article
- Global γH2AX phosphorylation inmicroPublication biology · 2025Article
- Dynamic protein assembly and architecture of the large solitary membraneless organelle during germline development in the wasp Nasonia vitripennis.Development (Cambridge, England) · 2024Article
- Analysis of meiotic recombination in Drosophila simulans shows no evidence of an interchromosomal effect.Genetics · 2024Article
- Transient caspase-mediated activation of caspase-activated DNase causes DNA damage required for phagocytic macrophage differentiation.Cell reports · 2024Article
- A transcriptomics-based RNAi screen for regulators of meiosis and early stages of oocyte development in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
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- Gap junction-transported cAMP from the niche controls stem cell progeny differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Heterozygous inversion breakpoints suppress meiotic crossovers by altering recombination repair outcomes.PLoS genetics · 2023Article
- Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.PLoS genetics · 2022Article
- <i>Drosophila</i> insulator proteins exhibit in vivo liquid-liquid phase separation properties.Life science alliance · 2022Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The recognition of DNA double-strand breaks (DSBs) using a phospho-specific antibody to the histone 2A variant has become the gold standard assay for DNA damage detection. Here we report on the development of the first monoclonal antibody to the phospho-specific form of Drosophila H2AV and characterize the specificity of this antibody to programmed DSBs in oocytes and rereplication sites in endocycling cells by immunofluorescence assays and to DSBs resulting from irradiation in both cell culture and whole tissue by Western blot assays. These studies show that the antibody derived in the study is highly specific for this modification that occurs at DSB sites, and therefore will be a new useful tool within the Drosophila community for the study of DNA damage response, DSB repair, meiotic recombination and chemical agents that cause DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.