ArticleThe Journal of biological chemistry2013
Formation of Arabidopsis Cryptochrome 2 photobodies in mammalian nuclei: application as an optogenetic DNA damage checkpoint switch.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- OptoLoop - an optogenetic tool to probe the functional role of genome organization.Journal of cell science · 2026Article
- OptoLoop: An optogenetic tool to probe the functional role of genome organization.bioRxiv : the preprint server for biology · 2025Article
- Light signaling in plants-a selective history.Plant physiology · 2024Review
- The Function and Photoregulatory Mechanisms of Cryptochromes From Moso Bamboo (Frontiers in plant science · 2022Article
- Structural insights into photoactivation of plant Cryptochrome-2.Communications biology · 2021Article
- Mechanisms of Cryptochrome-Mediated Photoresponses in Plants.Annual review of plant biology · 2020Article
- Photooligomerization Determines Photosensitivity and Photoreactivity of Plant Cryptochromes.Molecular plant · 2020Article
- Photodimerization systems for regulating protein-protein interactions with light.Current opinion in structural biology · 2019Review
- LADL: light-activated dynamic looping for endogenous gene expression control.Nature methods · 2019Article
- Beyond the photocycle-how cryptochromes regulate photoresponses in plants?Current opinion in plant biology · 2018Review
- Coactivator condensation at super-enhancers links phase separation and gene control.Science (New York, N.Y.) · 2018Article
- Overexpression of sweet sorghum cryptochrome 1a confers hypersensitivity to blue light, abscisic acid and salinity in Arabidopsis.Plant cell reports · 2018Article
- Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2.Nucleic acids research · 2017Article
- Engineering genetically-encoded tools for optogenetic control of protein activity.Current opinion in chemical biology · 2017Review
- Segmental duplications and evolutionary acquisition of UV damage response in the SPATA31 gene family of primates and humans.BMC genomics · 2017Article
- Cryptochromes Orchestrate Transcription Regulation of Diverse Blue Light Responses in Plants.Photochemistry and photobiology · 2017Review
- Bringing It All Together: Coupling Excision Repair to the DNA Damage Checkpoint.Photochemistry and photobiology · 2017Review
- Photoactivation and inactivation of Arabidopsis cryptochrome 2.Science (New York, N.Y.) · 2016Article
- The Blue Light-Dependent Polyubiquitination and Degradation of Arabidopsis Cryptochrome2 Requires Multiple E3 Ubiquitin Ligases.Plant & cell physiology · 2016Article
- Modular engineering of cellular signaling proteins and networks.Current opinion in structural biology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Nuclear bodies are discrete suborganelle structures that perform specialized functions in eukaryotic cells. In plant cells, light can induce de novo formation of nuclear bodies called photobodies (PBs) composed of the photosensory pigments, phytochrome (PHY) or cryptochrome (CRY). The mechanisms of formation, the exact compositions, and the functions of plant PBs are not known. Here, we have expressed Arabidopsis CRY2 (AtCRY2) in mammalian cells and analyzed its fate after blue light exposure to understand the requirements for PB formation, the functions of PBs, and their potential use in cell biology. We found that light efficiently induces AtCRY2-PB formation in mammalian cells, indicating that, other than AtCRY2, no plant-specific proteins or nucleic acids are required for AtCRY2-PB formation. Irradiation of AtCRY2 led to its degradation; however, degradation was not dependent upon photobody formation. Furthermore, we found that AtCRY2 photobody formation is associated with light-stimulated interaction with mammalian COP1 E3 ligase. Finally, we demonstrate that by fusing AtCRY2 to the TopBP1 DNA damage checkpoint protein, light-induced AtCRY2 PBs can be used to activate DNA damage signaling pathway in the absence of DNA damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.