ArticleThe Journal of antimicrobial chemotherapy2013
Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis.
Article in The Journal of antimicrobial chemotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 80 citations in OpenAlex.
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- The interplay of specialized metabolites, antibiotic resistance, and virulence in Enterococcus faecium: in silico analysis of bacterial genomes.BMC genomics · 2026Article
- A Daptomycin Resistance-Conferring cls1 I269T Mutation in Enterococcus faecium and its Global Prevalence.Current microbiology · 2026Article
- Selection and maintenance of mobile linezolid-resistance genes and plasmids carrying them in the presence of florfenicol, an animal-specific antimicrobial.Access microbiology · 2025Article
- Genetic Characterization of a Linezolid- and Penicillin-ResistantTransboundary and emerging diseases · 2025Article
- An antiplasmid system drives antibiotic resistance gene integration in carbapenemase-producing Escherichia coli lineages.Nature communications · 2024Article
- Antibiotic heteroresistance and persistence: an additional aid in hospital acquired infections byFuture microbiology · 2024Review
- The role of the universal sugar transport system components PtsI (EI) and PtsH (HPr) inFEMS microbes · 2024Article
- Recombination as an enforcement mechanism of prosocial behavior in cooperating bacteria.iScience · 2023Article
- Segregational drift hinders the evolution of antibiotic resistance on polyploid replicons.PLoS genetics · 2023Article
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- Vancomycin Resistance inMicroorganisms · 2022Review
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- A Small Multihost Plasmid CarryingFrontiers in veterinary science · 2022Article
- Segregational Drift Constrains the Evolutionary Rate of Prokaryotic Plasmids.Molecular biology and evolution · 2021Article
- Let Me Upgrade You: Impact of Mobile Genetic Elements on Enterococcal Adaptation and Evolution.Journal of bacteriology · 2021Review
- Piggybacking on Niche Adaptation Improves the Maintenance of Multidrug-Resistance Plasmids.Molecular biology and evolution · 2021Article
- Alternative vanHAX promoters and increased vanA-plasmid copy number resurrect silenced glycopeptide resistance in Enterococcus faecium.The Journal of antimicrobial chemotherapy · 2021Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo determine the fitness effects of various mobile genetic elements (MGEs) in Enterococcus faecium and Enterococcus faecalis when newly acquired. We also tested the hypothesis that the biological cost of vancomycin resistance plasmids could be mitigated during continuous growth in the laboratory.
methodsDifferent MGEs, including two conjugative transposons (CTns) of the Tn916 family (18 and 33 kb), a pathogenicity island (PAI) of 200 kb and vancomycin-resistance (vanA) plasmids (80-200 kb) of various origins and classes, were transferred into common ancestral E. faecium and E. faecalis strains by conjugation assays and experimentally evolved (vanA plasmids only). Transconjugants were characterized by PFGE, S1 nuclease assays and Southern blotting hybridization analyses. Single specific primer PCR was performed to determine the target sites for the insertion of the CTns. The fitness costs of various MGEs in E. faecium and E. faecalis were estimated in head-to-head competition experiments, and evolved populations were generated in serial transfer assays.
resultsThe biological cost of a newly acquired PAI and two CTns were both host- and insertion-locus-dependent. Newly acquired vanA plasmids may severely reduce host fitness (25%-27%), but these costs were rapidly mitigated after only 400 generations of continuous growth in the absence of antibiotic selection.
conclusionsNewly acquired MGEs may impose an immediate biological cost in E. faecium. However, as demonstrated for vanA plasmids, the initial costs of MGE carriage may be mitigated during growth and beneficial plasmid-host association can rapidly emerge.
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