ArticleThe Laryngoscope2014
A novel in vivo model for evaluating functional restoration of a tissue-engineered salivary gland.
Article in The Laryngoscope, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 60 citations in OpenAlex.
- Assessing bioactivity and biointegration of engineered salivary tissue constructs in a preclinical unilateral fractionated irradiated rat model.Acta biomaterialia · 2026Article
- Optimizing Primary Human Salivary Stem/Progenitor Cells for Tissue Engineering Applications.bioRxiv : the preprint server for biology · 2026Article
- The nerve center of organ engineering.Nature communications · 2025Review
- Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics.Journal of experimental & clinical cancer research : CR · 2024Article
- Trends of regenerative tissue engineering for oral and maxillofacial reconstruction in veterinary medicine.Frontiers in veterinary science · 2024Review
- Revolutionising oral organoids with artificial intelligence.Biomaterials translational · 2024Review
- Salivary Gland Bioengineering.Bioengineering (Basel, Switzerland) · 2023Review
- Microfluidic coaxial 3D bioprinting of cell-laden microfibers and microtubes for salivary gland tissue engineering.Biomaterials advances · 2023Article
- Matrix Degradability Contributes to the Development of Salivary Gland Progenitor Cells with Secretory Functions.ACS applied materials & interfaces · 2023Article
- Salivary gland function, development, and regeneration.Physiological reviews · 2022Review
- Bioengineering in salivary gland regeneration.Journal of biomedical science · 2022Review
- Identification of functional pathways for regenerative bioactivity of selected renal cells.Stem cell research & therapy · 2022Article
- Article
- Organoid Models for Salivary Gland Biology and Regenerative Medicine.Stem cells international · 2021Review
- Laminin-1 Peptides Conjugated to Fibrin Hydrogels Promote Salivary Gland Regeneration in Irradiated Mouse Submandibular Glands.Frontiers in bioengineering and biotechnology · 2021Article
- Immunosuppressed Miniswine as a Model for Testing Cell Therapy Success: Experience With Implants of Human Salivary Stem/Progenitor Cell Constructs.Frontiers in molecular biosciences · 2021Review
- Article
- Building a Functional Salivary Gland for Cell-Based Therapy: More than Secretory Epithelial Acini.Tissue engineering. Part A · 2020Review
- Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated αFrontiers in cell and developmental biology · 2019Article
- 2D and 3D cell cultures - a comparison of different types of cancer cell cultures.Archives of medical science : AMS · 2018Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
objectives/hypothesisTo create a novel model for development of a tissue-engineered salivary gland from human salivary gland cells that retains progenitor cell markers useful for treatment of radiation-induced xerostomia. STUDY
designA three-dimensional (3D) hyaluronic acid (HA)-based hydrogel scaffold was used to encapsulate primary human salivary gland cells and to obtain organized acini-like spheroids. Hydrogels were implanted into rat models, and cell viability and receptor expression were evaluated.
methodsA parotid gland surgical resection model for xenografting was developed. Salivary cells loaded in HA hydrogels formed spheroids and in vitro were implanted in the three-fourths resected parotid bed of athymic rats. Implants were removed after 1 week and analyzed for spheroid viability and phenotype retention.
resultsSpheroids in 3D stained positive for HA receptors CD168/RHAMM and CD44, which is also a progenitor cell marker. The parotid gland three-fourths resection model was well-tolerated by rodent hosts, and the salivary cell/hydrogel scaffolds were adherent to the remaining parotid gland, with no obvious signs of inflammation. A majority of human cells in the extracted hydrogels demonstrated robust expression of CD44.
conclusionsA 3D HA-based hydrogel scaffold that supported long-term culture of salivary gland cells into organized spheroids was established. An in vivo salivary gland resection model was developed that allowed for integration of the 3D HA hydrogel scaffold with the existing glandular parenchyma. The expression of CD44 among salivary cultures may partially explain their regenerative potential, and the expression of CD168/RHAMM along with CD44 may aid the development of these 3D spheroids into regenerated salivary glands. LEVEL OF EVIDENCE: NA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.