ArticleParasitology research2013
High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato.
Article in Parasitology research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The global status and genetic characterization of hydatidosis in camels (Parasitology · 2021Pooled it
- Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, ChinaPathogens (Basel, Switzerland) · 2023Article
- Prevalence of Cystic Echinococcosis Genotypes in Iranian Animals: A Systematic Review and Meta-Analysis.Journal of parasitology research · 2022Review
- Molecular Characterization and Haplotype Analyses of Lung Hydatid Cyst Isolates of Cattle and First Report of Echinococcus canadensis (G6/G7) in Cattle Isolates in Turkey.Acta parasitologica · 2021Article
- Molecular survey on cattle and sheep hydatidosis and first detection ofParasitology · 2020Article
- Cystic Echinococcosis of Camels: 12S rRNA Gene Variation Revealed Changing Pattern of Genetic Diversity WithinFrontiers in veterinary science · 2020Article
- Genetic survey of cystic echinococcosis in farm animals in Oman.Tropical animal health and production · 2020Article
- Genetic characterization ofParasitology · 2019Article
- Epidemiological and molecular studies onVeterinary world · 2019Article
- A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis.Gastroenterology and hepatology from bed to bench · 2016Article
- High-resolution melting analysis (HRM) for differentiation of four major Taeniidae species in dogs Taenia hydatigena, Taenia multiceps, Taenia ovis, and Echinococcus granulosus sensu stricto.Parasitology research · 2016Article
- Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences.Parasitology research · 2015Article
- Genetic diversity of Echinococcus granulosus in center of Iran.The Korean journal of parasitology · 2014Article
- Echinococcus granulosus genotypes in Iran.Gastroenterology and hepatology from bed to bench · 2014Review
- Rapid detection of Echinococcus species by a high-resolution melting (HRM) approach.Parasites & vectors · 2013Article
- Molecular Characterization ofIranian journal of parasitologyArticle
- Evaluation of Microsatellites Markers to Discriminate Four Main Taeniid Tapeworms of Dogs.Iranian journal of parasitologyArticle
- Genetic Identification ofIranian journal of parasitologyArticle
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reliable and rapid genotyping of large number of Echinococcus granulosus sensu lato isolates is crucial for understanding the epidemiology and transmission of cystic echinococcosis. We have developed a method for distinguishing and discriminating common genotypes of E. granulosus s.l. (G1, G3, and G6) in Iran. This method is based on polymerase chain reaction coupled with high resolution melting curve (HRM), ramping from 70 to 86 °C with fluorescence data acquisition set at 0.1 °C increments and continuous fluorescence monitoring. Consistency of this technique was assessed by inter- and intra-assays. Assessment of intra- and inter-assay variability showed low and acceptable coefficient of variations ranging from 0.09 to 0.17 %. Two hundred and eighty E. granulosus s.l. isolates from sheep, cattle, and camel were used to evaluate the applicability and accuracy of the method. The isolates were categorized as G1 (93, 94, and 25%), G3 (7, 4, and 4%), and G6 (0, 2, and 71%) for sheep, cattle, and camel, respectively. HRM results were completely compatible with those obtained from sequencing and rostellar hook measurement. This method proved to be a valuable screening tool for large-scale molecular epidemiological studies.
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