ArticleIntegrative biology : quantitative biosciences from nano to macro2013
Transforming potential and matrix stiffness co-regulate confinement sensitivity of tumor cell migration.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Gradients in cell density and shape transitions drive collective cell migration into confining environments.Soft matter · 2025Article
- Linking cell mechanical memory and cancer metastasis.Nature reviews. Cancer · 2024Review
- The integration of bulk and single-cell sequencing data revealed the function of FKBP10 in the gastric cancer microenvironment.Translational cancer research · 2024Article
- Matrix obstructions cause multiscale disruption in collective epithelial migration by suppressing leader cell function.Molecular biology of the cell · 2023Article
- Matrix stiffness regulates tumor cell intravasation through expression and ESRP1-mediated alternative splicing of MENA.Cell reports · 2023Article
- Diabetic hyperglycemia promotes primary tumor progression through glycation-induced tumor extracellular matrix stiffening.Science advances · 2022Article
- Migration and 3D Traction Force Measurements inside Compliant Microchannels.Nano letters · 2022Article
- Nucleoli in epithelial cell collectives respond to tumorigenic, spatial, and mechanical cues.Molecular biology of the cell · 2022Article
- Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition.Cancers · 2022Article
- Regulation of Tumor Invasion by the Physical Microenvironment: Lessons from Breast and Brain Cancer.Annual review of biomedical engineering · 2022Review
- Review
- Evaluation of Cell's Passability in the ECM Network.Biophysical journal · 2020Article
- Unjamming and collective migration in MCF10A breast cancer cell lines.Biochemical and biophysical research communications · 2020Article
- Controlled dissolution of freeform 3D printed carbohydrate glass scaffolds in hydrogels using a hydrophobic spray coating.Additive manufacturing · 2019Article
- Predicting Collective Migration of Cell Populations Defined by Varying Repolarization Dynamics.Biophysical journal · 2018Article
- Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory.Biomaterials · 2017Article
- Effects of Liposomes Contained in Thermosensitive Hydrogels as Biomaterials Useful in Neural Tissue Engineering.Materials (Basel, Switzerland) · 2017Article
- Recapitulating the human tumor microenvironment: Colon tumor-derived extracellular matrix promotes angiogenesis and tumor cell growth.Biomaterials · 2017Article
- Cancer cell motility: lessons from migration in confined spaces.Nature reviews. Cancer · 2017Review
- Scattering of Cell Clusters in Confinement.Biophysical journal · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
It is now well established that tumor cell invasion through tissue is strongly regulated by the microstructural and mechanical properties of the extracellular matrix (ECM). However, it remains unclear how these physical microenvironmental inputs are jointly processed with oncogenic lesions to drive invasion. In this study, we address this open question by combining a microfabricated polyacrylamide channel (μPAC) platform that enables independent control of ECM stiffness and confinement with an isogenically-matched breast tumor progression series in which the oncogenes ErbB2 and 14-3-3ζ are overexpressed independently or in tandem. We find that increasing channel confinement and overexpressing ErbB2 both promote cell migration to a similar degree when other parameters are kept constant. In contrast, 14-3-3ζ overexpression slows migration speed, and does so in a fashion that dwarfs effects of ECM confinement and stiffness. We also find that ECM stiffness dramatically enhances cell motility when combined with ErbB2 overexpression, demonstrating that biophysical cues and cell-intrinsic parameters promote cell invasion in an integrative manner. Morphometric analysis of cells inside the μPAC platform reveals that the rapid cell migration induced by narrow channels and ErbB2 overexpression are both accompanied by increased cell polarization. Disruption of this polarization occurs by pharmacological inhibition of Rac GTPase phenocopies 14-3-3ζ overexpression by reducing cell polarization and slowing migration. By systematically measuring migration speed as a function of matrix stiffness and confinement, we also quantify for the first time the sensitivity of migration speed to microchannel properties and transforming potential. These results demonstrate that oncogenic lesions and ECM biophysical properties can synergistically interact to drive invasive migration, and that both inputs may act through common molecular mechanisms to enhance migration speed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.