ArticleAmerican journal of physiology. Lung cellular and molecular physiology2013
DNA methylation in nasal epithelial cells from smokers: identification of ULBP3-related effects.
Article in American journal of physiology. Lung cellular and molecular physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Effect of broccoli sprouts on nasal response to live attenuated influenza virus in smokers: a randomized, double-blind study.PloS one · 2014Trial
- Development of the InTelligence And Machine LEarning (TAME) Toolkit for Introductory Data Science, Chemical-Biological Analyses, Predictive Modeling, and Database Mining for Environmental Health Research.Frontiers in toxicology · 2022Article
- Impact of inhaled pollutants on response to viral infection in controlled exposures.The Journal of allergy and clinical immunology · 2021Review
- Differential responses to e-cig generated aerosols from humectants and different forms of nicotine in epithelial cells from nonsmokers and smokers.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Use of Electronic Cigarettes and Self-Reported Chronic Obstructive Pulmonary Disease Diagnosis in Adults.Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco · 2020Article
- New Approach Methods to Evaluate Health Risks of Air Pollutants: Critical Design Considerations for In Vitro Exposure Testing.International journal of environmental research and public health · 2020Review
- Understanding the Early Biological Effects of Isoprene-Derived Particulate Matter Enhanced by Anthropogenic Pollutants.Research report (Health Effects Institute) · 2019Article
- Tobacco biomarkers and genetic/epigenetic analysis to investigate ethnic/racial differences in lung cancer risk among smokers.NPJ precision oncology · 2018Review
- Flavored e-cigarette liquids and cinnamaldehyde impair respiratory innate immune cell function.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Integrating mean and variance heterogeneities to identify differentially expressed genes.BMC bioinformatics · 2016Article
- E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Article
- Aberrant DNA Methylation of Phosphodiesterase [corrected] 4D Alters Airway Smooth Muscle Cell Phenotypes.American journal of respiratory cell and molecular biology · 2016Article
- Will chronic e-cigarette use cause lung disease?American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Attenuated Airway Epithelial Cell Interleukin-22R1 Expression in the Infant Nonhuman Primate Lung.American journal of respiratory cell and molecular biology · 2015Article
- Epigenetics and Preeclampsia: Defining Functional Epimutations in the Preeclamptic Placenta Related to the TGF-β Pathway.PloS one · 2015Article
- A critical review of the American Journal of Physiology-Lung Cellular and Molecular Physiology: 2012-2015.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- Epigenetic epidemiology: promises for public health research.Environmental and molecular mutagenesis · 2014Review
- Regulation and activity of secretory leukoprotease inhibitor (SLPI) is altered in smokers.American journal of physiology. Lung cellular and molecular physiology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We previously demonstrated that, in nasal epithelial cells (NECs) from smokers, methylation of an antiviral gene was associated with impaired antiviral defense responses. To expand these findings and better understand biological mechanisms underlying cigarette smoke (CS)-induced modifications of host defense responses, we aimed to compare DNA methylation of genes that may play a role in antiviral response. We used a two-tiered analytical approach, where we first implemented a genome-wide strategy. NECs from smokers differed in the methylation levels of 390 genes, the majority (84%) of which showed decreased methylation in smokers. Secondly, we generated an a priori set of 161 antiviral response-related genes, of which five were differentially methylated in NEC from smokers (CCL2, FDPS, GSK3B, SOCS3, and ULBP3). Assessing these genes at the systems biology level revealed a protein interaction network associated with CS-induced epigenetic modifications involving SOCS3 and ULBP3 signaling, among others. Subsequent confirmation studies focused on SOCS3 and ULBP3, which were hypomethylated and hypermethylated, respectively. Expression of SOCS3 was increased, whereas ULBP3 expression was decreased in NECs from smokers. Addition of the demethylating agent 5-Aza-2-deoxycytidine enhanced ULBP3 expression in NECs from smokers. Furthermore, infection of differentiated NECs with influenza virus resulted in significantly lower levels of ULBP3 in cells from smokers. Taken together, our findings show that genomic DNA methylation profiles are altered in NECs from smokers and that these changes are associated with decreased antiviral host defense responses, indicating that epigenenic dysregulation of genes such as SOCS3 and ULBP3 likely impacts immune responses in the epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.