ArticleMetabolism: clinical and experimental2013
Exercise training does not increase muscle FNDC5 protein or mRNA expression in pigs.
Article in Metabolism: clinical and experimental, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Sex specific effects of irisin on the skeleton.Bone research · 2026Review
- Physical activity-induced myokine responses in major mammalian farm animal species: a mini-review.Frontiers in veterinary science · 2026Review
- Housing temperature influences exercise-induced glucose regulation and expression of exerkines in mice.Experimental physiology · 2025Article
- Highlighting the novel effects of high-intensity interval training on some histopathological and molecular indices in the heart of type 2 diabetic rats.Frontiers in endocrinology · 2023Article
- Irisin: A New Code Uncover the Relationship of Skeletal Muscle and Cardiovascular Health During Exercise.Frontiers in physiology · 2021Review
- Concentrations of Circulating Irisin and Myostatin in Race and Endurace Purebred Arabian Horses-Preliminary Study.Animals : an open access journal from MDPI · 2020Article
- Guidelines for animal exercise and training protocols for cardiovascular studies.American journal of physiology. Heart and circulatory physiology · 2020Review
- Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α.Eating and weight disorders : EWD · 2020Article
- A transcriptomic study of myogenic differentiation under the overexpression of PPARγ by RNA-Seq.Scientific reports · 2017Article
- Secretome profiling of oral squamous cell carcinoma-associated fibroblasts reveals organization and disassembly of extracellular matrix and collagen metabolic process signatures.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Circulating irisin levels and muscle FNDC5 mRNA expression are independent of IL-15 levels in mice.Endocrine · 2015Article
- New research developments and insights from Metabolism.Metabolism: clinical and experimental · 2015Article
- Plasma irisin in runners and nonrunners: no favorable metabolic associations in humans.Physiological reports · 2015Article
- Circulating irisin levels and coronary heart disease: association with future acute coronary syndrome and major adverse cardiovascular events.International journal of obesity (2005) · 2015Article
- Diet quality is associated with circulating C-reactive protein but not irisin levels in humans.Metabolism: clinical and experimental · 2014Article
- Irisin is elevated in skeletal muscle and serum of mice immediately after acute exercise.International journal of biological sciences · 2014Article
- Article
- Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?PloS one · 2014Article
- Irisin mRNA and circulating levels in relation to other myokines in healthy and morbidly obese humans.European journal of endocrinology · 2013Observational
- Circulating irisin in relation to insulin resistance and the metabolic syndrome.The Journal of clinical endocrinology and metabolism · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundExercise training elevates circulating irisin and induces the expression of the FNDC5 gene in skeletal muscles of mice. Our objective was to determine whether exercise training also increases FNDC5 protein or mRNA expression in the skeletal muscles of pigs as well as plasma irisin.
methodsCastrated male pigs of the Rapacz familial hypercholesterolemic (FHM) strain and normal (Yucatan miniature) pigs were sacrificed after 16-20 weeks of exercise training. Samples of cardiac muscle, deltoid and triceps brachii muscle, subcutaneous and epicardial fat were obtained and FNDC5 mRNA, along with that of 6 other genes, was measured in all tissues of FHM pigs by reverse transcription polymerase chain reaction. FNDC protein in deltoid and triceps brachii was determined by Western blotting in both FHM and normal pigs. Citrate synthase activity was measured in the muscle samples of all pigs as an index of exercise training. Irisin was measured by an ELISA assay.
resultsThere was no statistically significant effect of exercise training on FNDC5 gene expression in epicardial or subcutaneous fat, deltoid muscle, triceps brachii muscle or heart muscle. Exercise-training elevated circulating levels of irisin in the FHM pigs and citrate synthase activity in deltoid and triceps brachii muscle. A similar increase in citrate synthase activity was seen in muscle extracts of exercise-trained normal pigs but there was no alteration in circulating irisin.
conclusionExercise training in pigs does not increase FNDC5 mRNA or protein in the deltoid or triceps brachii of FHM or normal pigs while increasing circulating irisin only in the FHM pigs. These data indicate that the response to exercise training in normal pigs is not comparable to that seen in mice.
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