ArticleCytotherapy2013
Mobilization of endogenous stem cell populations enhances fracture healing in a murine femoral fracture model.
Article in Cytotherapy, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Pooled it
- Rapid and selective mobilization of specific stem cell types after consumption of a polyphenol-rich extract from sea buckthorn berries (Clinical interventions in aging · 2019Trial
- Protective Effect of L-Arginine and Filgrastim Against Valproic Acid-Induced Histopathological Changes on the Gastric and Lingual Mucosa.Journal of applied toxicology : JAT · 2026Article
- Endogenous stem cell mobilization and localized immunosuppression synergistically ameliorate DSS-induced Colitis in mice.Stem cell research & therapy · 2024Article
- Current and future uses of skeletal stem cells for bone regeneration.World journal of stem cells · 2020Review
- CXCR4 Antagonism to Treat Delayed Fracture Healing.Tissue engineering. Part A · 2019Article
- VEGF with AMD3100 endogenously mobilizes mesenchymal stem cells and improves fracture healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Circulating progenitor cells and the expression of Cxcl12, Cxcr4 and angiopoietin-like 4 during wound healing in the murine ear.PloS one · 2019Article
- Bone marrow cell homing to sites of acute tibial fracture:EJNMMI research · 2018Article
- Combination therapy with BMP-2 and psoralen enhances fracture healing in ovariectomized mice.Experimental and therapeutic medicine · 2018Article
- Novel Lipid Signaling Mediators for Mesenchymal Stem Cell Mobilization during Bone Repair.Cellular and molecular bioengineering · 2018Article
- Bone regeneration in the stem cell era: safe play for the patient?Clinical rheumatology · 2017Review
- Trauma and Stem Cells: Biology and Potential Therapeutic Implications.International journal of molecular sciences · 2017Review
- Prospective Review of Mesenchymal Stem Cells Differentiation into Osteoblasts.Orthopaedic surgery · 2017Review
- Stem and progenitor cells: advancing bone tissue engineering.Drug delivery and translational research · 2016Review
- Changing Paradigms in Cranio-Facial Regeneration: Current and New Strategies for the Activation of Endogenous Stem Cells.Frontiers in physiology · 2016Review
- Bioactive lipid coating of bone allografts directs engraftment and fate determination of bone marrow-derived cells in rat GFP chimeras.Biomaterials · 2015Article
- Strategies to Stimulate Mobilization and Homing of Endogenous Stem and Progenitor Cells for Bone Tissue Repair.Frontiers in bioengineering and biotechnology · 2015Review
- The convergence of fracture repair and stem cells: interplay of genes, aging, environmental factors and disease.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Review
- AMD3100, a stem cell mobilizer, aids fracture healing in mice.BoneKEy reports · 2013Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
BACKGROUND
aimsDelivery of bone marrow-derived stem and progenitor cells to the site of injury is an effective strategy to enhance bone healing. An alternate approach is to mobilize endogenous, heterogeneous stem cells that will home to the site of injury. AMD3100 is an antagonist of the chemokine receptor 4 (CXCR4) that rapidly mobilizes stem cell populations into peripheral blood. Our hypothesis was that increasing circulating numbers of stem and progenitor cells using AMD3100 will improve bone fracture healing.
methodsA transverse femoral fracture was induced in C57BL/6 mice, after which they were subcutaneously injected for 3 d with AMD3100 or saline control. Mesenchymal stromal cells, hematopoietic stem and progenitor cells and endothelial progenitor cells in the peripheral blood and bone marrow were evaluated by means of flow cytometry, automated hematology analysis and cell culture 24 h after injection and/or fracture. Healing was assessed up to 84 d after fracture by histomorphometry and micro-computed tomography.
resultsAMD3100 injection resulted in higher numbers of circulating mesenchymal stromal cells, hematopoietic stem cells and endothelial progenitor cells. Micro-computed tomography data demonstrated that the fracture callus was significantly larger compared with the saline controls at day 21 and significantly smaller (remodeled) at day 84. AMD3100-treated mice have a significantly higher bone mineral density than do saline-treated counterparts at day 84.
conclusionsOur data demonstrate that early cell mobilization had significant positive effects on healing throughout the regenerative process. Rapid mobilization of endogenous stem cells could provide an effective alternative strategy to cell transplantation for enhancing tissue regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.