ArticleAnalytical chemistry2013
MALDI imaging and in situ identification of integral membrane proteins from rat brain tissue sections.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Relative Quantitation of EFNA1 Expression in Mouse Heart Tissue Histologic Sections Using MALDI-MSI.International journal of molecular sciences · 2025Article
- Review
- Brain and Serum Membrane Vesicle (Exosome) Profiles in Experimental Alcohol-Related Brain Degeneration: Forging the Path to Non-Invasive Liquid Biopsy Diagnostics.Journal of molecular pathology · 2024Article
- Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue.Journal of the American Chemical Society · 2023Article
- Rapid Multivariate Analysis Approach to Explore Differential Spatial Protein Profiles in Tissue.Journal of proteome research · 2023Article
- Automated annotation and visualisation of high-resolution spatial proteomic mass spectrometry imaging data using HIT-MAP.Nature communications · 2021Article
- Protein identification strategies in MALDI imaging mass spectrometry: a brief review.Current opinion in chemical biology · 2019Review
- DBDA as a Novel Matrix for the Analyses of Small Molecules and Quantification of Fatty Acids by Negative Ion MALDI-TOF MS.Journal of the American Society for Mass Spectrometry · 2018Article
- Anatomical-Molecular Distribution of EphrinA1 in Infarcted Mouse Heart Using MALDI Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2018Article
- Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.Journal of proteome research · 2018Article
- Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve.Biochimica et biophysica acta. Proteins and proteomics · 2017Review
- A Proof of Concept to Bridge the Gap between Mass Spectrometry Imaging, Protein Identification and Relative Quantitation: MSI~LC-MS/MS-LF.Proteomes · 2016Article
- Oligodendroglial membrane dynamics in relation to myelin biogenesis.Cellular and molecular life sciences : CMLS · 2016Review
- Automated Protein Localization of Blood Brain Barrier Vasculature in Brightfield IHC Images.PloS one · 2016Article
- Differential Contributions of Alcohol and Nicotine-Derived Nitrosamine Ketone (NNK) to White Matter Pathology in the Adolescent Rat Brain.Alcohol and alcoholism (Oxford, Oxfordshire) · 2015Article
- Decellularization of intact tissue enables MALDI imaging mass spectrometry analysis of the extracellular matrix.Journal of mass spectrometry : JMS · 2015Article
- Histology-directed and imaging mass spectrometry: An emerging technology in ectopic calcification.Bone · 2015Article
- Imaging mass spectrometry for assessing cutaneous wound healing: analysis of pressure ulcers.Journal of proteome research · 2015Article
- Histology-directed microwave assisted enzymatic protein digestion for MALDI MS analysis of mammalian tissue.Analytical chemistry · 2015Article
- On-tissue localization of ceramides and other sphingolipids by MALDI mass spectrometry imaging.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transmembrane proteins are greatly underrepresented in data generated by imaging mass spectrometry (IMS) because of analytical challenges related to their size and solubility. Here, we present the first example of MALDI IMS of two highly modified multitransmembrane domain proteins, myelin proteolipid protein (PLP, 30 kDa) and DM-20 (26 kDa), from various regions of rat brain, namely, the cerebrum, cerebellum, and medulla. We utilize a novel tissue pretreatment aimed at transmembrane protein enrichment to show the in situ distribution of fatty acylation of these proteins, particularly of post-translational palmitoylation. Additionally, we demonstrate the utility of protease-encapsulated hydrogels for spatially localized on-tissue protein digestion and peptide extraction for subsequent direct coupling to LC-MS/MS for protein identification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.