ArticleMolecular & cellular proteomics : MCP2013
Systems-level overview of host protein phosphorylation during Shigella flexneri infection revealed by phosphoproteomics.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Profiling Selectivity for theBiochemistry · 2026Article
- The Shigella kinase effector OspG modulates host ubiquitin signaling to escape septin-cage entrapment.Nature communications · 2024Article
- Unveiling signaling pathways inducing MHC class II expression in neutrophils.Frontiers in immunology · 2024Article
- Roles of host SUMOylation in bacterial pathogenesis.Infection and immunity · 2023Review
- The phosphoproteome of choroid plexus epithelial cells following infection with Neisseria meningitidis.Frontiers in cellular and infection microbiology · 2023Article
- Phosphoproteomics data-driven signalling network inference: Does it work?Computational and structural biotechnology journal · 2023Article
- LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome.Cell · 2021Article
- Bioinformatic Analysis of Temporal and Spatial Proteome Alternations During Infections.Frontiers in genetics · 2021Review
- The T3SS ofMicroorganisms · 2020Review
- Bacterial virulence mediated by orthogonal post-translational modification.Nature chemical biology · 2020Review
- Evidence for a role of protein phosphorylation in the maintenance of the cnidarian-algal symbiosis.Molecular ecology · 2019Article
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- Transcriptomics analysis of host liver and meta-transcriptome analysis of rumen epimural microbial community in young calves treated with artificial dosing of rumen content from adult donor cow.Scientific reports · 2019Article
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- Quantitative proteomic and phosphoproteomic comparison of human colon cancer DLD-1 cells differing in ploidy and chromosome stability.Molecular biology of the cell · 2018Article
- The Tyrosine Kinase Inhibitor Gefitinib Restricts Mycobacterium tuberculosis Growth through Increased Lysosomal Biogenesis and Modulation of Cytokine Signaling.ACS infectious diseases · 2017Article
- Global Reprogramming of Host Kinase Signaling in Response to Fungal Infection.Cell host & microbe · 2017Article
- ALPK1 controls TIFA/TRAF6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria.PLoS pathogens · 2017Article
- Global Proteomics RevealedFrontiers in cellular and infection microbiology · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enteroinvasive bacterium Shigella flexneri invades the intestinal epithelium of humans. During infection, several injected effector proteins promote bacterial internalization, and interfere with multiple host cell responses. To obtain a systems-level overview of host signaling during infection, we analyzed the global dynamics of protein phosphorylation by liquid chromatography-tandem MS and identified several hundred of proteins undergoing a phosphorylation change during the first hours of infection. Functional bioinformatic analysis revealed that they were mostly related to the cytoskeleton, transcription, signal transduction, and cell cycle. Fuzzy c-means clustering identified six temporal profiles of phosphorylation and a functional module composed of ATM-phosphorylated proteins related to genotoxic stress. Pathway enrichment analysis defined mTOR as the most overrepresented pathway. We showed that mTOR complex 1 and 2 were required for S6 kinase and AKT activation, respectively. Comparison with a published phosphoproteome of Salmonella typhimurium-infected cells revealed a large subset of coregulated phosphoproteins. Finally, we showed that S. flexneri effector OspF affected the phosphorylation of several hundred proteins, thereby demonstrating the wide-reaching impact of a single bacterial effector on the host signaling network.
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