ArticleProtoplasma2014
Selective binding of lectins to normal and neoplastic urothelium in rat and mouse bladder carcinogenesis models.
Article in Protoplasma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Sensing of cardiolipin exposure on plasma membranes of apoptotic cells by EryA-mCherry protein.The FEBS journal · 2026Article
- Urinary Bladder Cancer Induced by N-Butyl-N-(4-Hydroxybutyl)-Nitrosamine.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Jacalin-Curcumin Complex Sensitizes the Breast Cancer MDA-MB-231 Cell Line.International journal of molecular sciences · 2023Article
- In focus in HCB.Histochemistry and cell biology · 2023Article
- Selective targeting of lectins and their macropinocytosis in urothelial tumours: translation from in vitro to ex vivo.Histochemistry and cell biology · 2023Article
- The Golgi complex: An organelle that determines urothelial cell biology in health and disease.Histochemistry and cell biology · 2022Review
- Nanodiamonds as Possible Tools for Improved Management of Bladder Cancer and Bacterial Cystitis.International journal of molecular sciences · 2022Review
- Lectins as Biomarkers of IC/BPS Disease: A Comparative Study of Glycosylation Patterns in Human Pathologic Urothelium and IC/BPS Experimental Models.Diagnostics (Basel, Switzerland) · 2022Article
- Cytotoxic Activity of LLO Y406A Is Targeted to the Plasma Membrane of Cancer Urothelial Cells.International journal of molecular sciences · 2021Article
- Combined lectin- and immuno-histochemistry (CLIH) for applications in cell biology and cancer diagnosis: Analysis of human urothelial carcinomas.European journal of histochemistry : EJH · 2020Article
- Jacalin-Activated Macrophages Exhibit an Antitumor Phenotype.BioMed research international · 2016Article
- Cell type-specific response to high intracellular loading of polyacrylic acid-coated magnetic nanoparticles.International journal of nanomedicine · 2015Article
- Glycosylation of uroplakins. Implications for bladder physiopathology.Glycoconjugate journal · 2014Review
- Recombinant production of plant lectins in microbial systems for biomedical application - the frutalin case study.Frontiers in plant science · 2014Review
- Animal models and therapeutic molecular targets of cancer: utility and limitations.Drug design, development and therapy · 2014Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bladder cancer adjuvant intravesical therapy could be optimized by more selective targeting of neoplastic tissue via specific binding of lectins to plasma membrane carbohydrates. Our aim was to establish rat and mouse models of bladder carcinogenesis to investigate in vivo and ex vivo binding of selected lectins to the luminal surface of normal and neoplastic urothelium. Male rats and mice were treated with 0.05 % N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) in drinking water and used for ex vivo and in vivo lectin binding experiments. Urinary bladder samples were also used for paraffin embedding, scanning electron microscopy and immunofluorescence labelling of uroplakins. During carcinogenesis, the structure of the urinary bladder luminal surface changed from microridges to microvilli and ropy ridges and the expression of urothelial-specific glycoproteins uroplakins was decreased. Ex vivo and in vivo lectin binding experiments gave comparable results. Jacalin (lectin from Artocarpus integrifolia) exhibited the highest selectivity for neoplastic compared to normal urothelium of rats and mice. The binding of lectin from Amaranthus caudatus decreased in rat model and increased in mouse carcinogenesis model, indicating interspecies variations of plasma membrane glycosylation. Lectin from Datura stramonium showed higher affinity for neoplastic urothelium compared to the normal in rat and mouse model. The BBN-induced animal models of bladder carcinogenesis offer a promising approach for lectin binding experiments and further lectin-mediated targeted drug delivery research. Moreover, in vivo lectin binding experiments are comparable to ex vivo experiments, which should be considered when planning and optimizing future research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.