ArticleScientific reports2013
Microsecond dynamics of an unfolded protein by a line confocal tracking of single molecule fluorescence.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Chronic constipation and the brain-gut-microbiome axis: the role of 5-HT signaling and Traditional Chinese Medicine in pathophysiology and treatment.Frontiers in medicine · 2025Review
- Quantifying Microsecond Solution-Phase Conformational Dynamics of a DNA Hairpin at the Single-Molecule Level.ACS physical chemistry Au · 2024Article
- Pulsed-Interleaved-Excitation Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy.The journal of physical chemistry. B · 2024Article
- Simple and Efficient Detection Scheme of Two-Color Fluorescence Correlation Spectroscopy for Protein Dynamics Investigation from Nanoseconds to Milliseconds.ACS physical chemistry Au · 2024Article
- The B domain of protein A retains residual structures in 6 M guanidinium chloride as revealed by hydrogen/deuterium-exchange NMR spectroscopy.Protein science : a publication of the Protein Society · 2023Article
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy: Concepts and Applications.Molecules (Basel, Switzerland) · 2018Review
- LinkingeLife · 2018Article
- Hypothesis: structural heterogeneity of the unfolded proteins originating from the coupling of the local clusters and the long-range distance distribution.Biophysical reviews · 2018Review
- 16-Ch Time-resolved Single-Molecule Spectroscopy Using Line Excitation.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
- Microsecond protein dynamics observed at the single-molecule level.Nature communications · 2015Article
- Real-time submillisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering.Nature communications · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a new method for high speed tracking of fluorescence time series from single proteins. The method uses a fast sample flow and a modified confocal microscopy, line confocal microscopy, and achieves the time resolution of less than 20 μs. The obtained time series from the B domain of protein A labeled with donor and acceptor fluorophores suggest conformational heterogeneity and dynamic fluctuations in the unfolded state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.