ArticleBiotechnology for biofuels2013
Transcriptome response to alkane biofuels in Saccharomyces cerevisiae: identification of efflux pumps involved in alkane tolerance.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 90 citations in OpenAlex.
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- Elucidating Bile Acid Tolerance inFoods (Basel, Switzerland) · 2024Article
- Multi-modular metabolic engineering and efflux engineering for enhanced lycopene production in recombinant Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2024Article
- Review
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- High-Titer Production of the Fungal Anhydrotetracycline, TAN-1612, in Engineered Yeasts.ACS synthetic biology · 2022Article
- Insights into cyanobacterial alkane biosynthesis.Journal of industrial microbiology & biotechnology · 2022Review
- Using genome-wide expression compendia to study microorganisms.Computational and structural biotechnology journal · 2022Review
- Microbial engineering to produce fatty alcohols and alkanes.Journal of industrial microbiology & biotechnology · 2021Review
- In-depth understanding of molecular mechanisms of aldehyde toxicity to engineer robust Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Review
- The Wonderful Activities of the GenusMolecules (Basel, Switzerland) · 2021Review
- Adaptation to Industrial Stressors Through Genomic and Transcriptional Plasticity in a Bioethanol Producing Fission Yeast Isolate.G3 (Bethesda, Md.) · 2020Article
- Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene inBiotechnology for biofuels · 2020Article
- Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals.Open biology · 2019Review
- Production of γ-terpinene by metabolically engineeredRSC advances · 2018Article
- The Paralogous GenesFrontiers in genetics · 2018Article
- Engineering tolerance to industrially relevant stress factors in yeast cell factories.FEMS yeast research · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydrocarbon alkanes have been recently considered as important next-generation biofuels because microbial production of alkane biofuels was demonstrated. However, the toxicity of alkanes to microbial hosts can possibly be a bottleneck for high productivity of alkane biofuels. To tackle this toxicity issue, it is essential to understand molecular mechanisms of interactions between alkanes and microbial hosts, and to harness these mechanisms to develop microbial host strains with improved tolerance against alkanes. In this study, we aimed to improve the tolerance of Saccharomyces cerevisiae, a model eukaryotic host of industrial significance, to alkane biofuels by exploiting cellular mechanisms underlying alkane response.
resultsTo this end, we first confirmed that nonane (C9), decane (C10), and undecane (C11) were significantly toxic and accumulated in S. cerevisiae. Transcriptome analyses suggested that C9 and C10 induced a range of cellular mechanisms such as efflux pumps, membrane modification, radical detoxification, and energy supply. Since efflux pumps could possibly aid in alkane secretion, thereby reducing the cytotoxicity, we formed the hypothesis that those induced efflux pumps could contribute to alkane export and tolerance. In support of this hypothesis, we demonstrated the roles of the efflux pumps Snq2p and Pdr5p in reducing intracellular levels of C10 and C11, as well as enhancing tolerance levels against C10 and C11. This result provided the evidence that Snq2p and Pdr5p were associated with alkane export and tolerance in S. cerevisiae.
conclusionsHere, we investigated the cellular mechanisms of S. cerevisiae response to alkane biofuels at a systems level through transcriptome analyses. Based on these mechanisms, we identified efflux pumps involved in alkane export and tolerance in S. cerevisiae. We believe that the results here provide valuable insights into designing microbial engineering strategies to improve cellular tolerance for highly efficient alkane biofuel production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.