ArticleBMC genomics2013
The Saccharomyces cerevisiae transcriptome as a mirror of phytochemical variation in complex extracts of Equisetum arvense from America, China, Europe and India.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives.Biomolecules · 2019Pooled it
- Evaluation of Morphological, Phytochemical, and Antioxidant Properties of WildInternational journal of analytical chemistry · 2026Article
- Plastid Genome ofPlants (Basel, Switzerland) · 2022Article
- Chemical composition and antifungal activity of plant extracts traditionally used in organic and biodynamic farming.Environmental science and pollution research international · 2018Article
- A multiple biomarker assay for quality assessment of botanical drugs using a versatile microfluidic chip.Scientific reports · 2017Article
- DNA Microarray-Based Screening and Characterization of Traditional Chinese Medicine.Microarrays (Basel, Switzerland) · 2017Review
- Application of multi-target phytotherapeutic concept in malaria drug discovery: a systems biology approach in biomarker identification.Biomarker research · 2016Review
- An in vitro study of anti-inflammatory activity of standardised Andrographis paniculata extracts and pure andrographolide.BMC complementary and alternative medicine · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPattern-oriented chemical profiling is increasingly being used to characterize the phytochemical composition of herbal medicines for quality control purposes. Ideally, a fingerprint of the biological effects should complement the chemical fingerprint. For ethical and practical reasons it is not possible to test each herbal extract in laboratory animals or humans. What is needed is a test system consisting of an organism with relevant biology and complexity that can serve as a surrogate in vitro system. The purpose of this study was to test the hypothesis that the Saccharomyces cerevisiae transcriptome might be used as an indicator of phytochemical variation of closely-related yet distinctly different extracts prepared from a single species of a phytogeographically widely distributed medicinal plant. We combined phytochemical profiling using chromatographic methods (HPTLC, HPLC-PDA-MS/MS) and gene expression studies using Affymetrix Yeast 2.0 gene chip with principal component analysis and k-nearest neighbor clustering analysis to test this hypothesis using extracts prepared from the phytogeographically widely distributed medicinal plant Equisetum arvense as a test case.
resultsWe found that the Equisetum arvense extracts exhibited qualitative and quantitative differences in their phytochemical composition grouped along their phytogeographical origin. Exposure of yeast to the extracts led to changes in gene expression that reflected both the similarities and differences in the phytochemical composition of the extracts. The Equisetum arvense extracts elicited changes in the expression of genes involved in mRNA translation, drug transport, metabolism of energy reserves, phospholipid metabolism, and the cellular stress response.
conclusionsOur data show that functional genomics in S. cerevisiae may be developed as a sensitive bioassay for the scientific investigation of the interplay between phytochemical composition and transcriptional effects of complex mixtures of chemical compounds. S. cerevisiae transcriptomics may also be developed for testing of mixtures of conventional drugs ("polypills") to discover novel antagonistic or synergistic effects of those drug combinations.
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