ArticleInternational journal of clinical and experimental pathology2013
Utility of fluorescence in situ hybridization to detect MDM2 amplification in liposarcomas and their morphological mimics.
Article in International journal of clinical and experimental pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 62 citations in OpenAlex.
- Droplet Digital PCR Assessment ofInternational journal of molecular sciences · 2026Article
- Real-world survival outcomes and MDM2 prevalence in US patients with metastatic dedifferentiated liposarcoma.Future oncology (London, England) · 2025Article
- Different Shades of Desmoid-Type Fibromatosis (DTF): Detection of Noval Mutations in the Clinicopathologic Analysis of 32 Cases.Diagnostics (Basel, Switzerland) · 2024Article
- Soft Tissue Sarcomas with Chromosomal Alterations in the 12q13-15 Region: Differential Diagnosis and Therapeutic Implications.Cancers · 2024Review
- MDM2 Implications for Potential Molecular Pathogenic Therapies of Soft-Tissue Tumors.Journal of clinical medicine · 2023Review
- FISH Diagnostic Assessment ofInternational journal of molecular sciences · 2023Article
- Molecular testing of soft tissue tumors.Diagnostic cytopathology · 2023Review
- In situ hybridization to detect DNA amplification in extracellular vesicles.Journal of extracellular vesicles · 2022Article
- Molecular classification of soft tissue sarcomas for adequate diagnosis: A study on the northeast population of Morocco.Heliyon · 2022Article
- Giant retroperitoneal liposarcoma treated with radical conservative surgery: A case report and review of literature.World journal of clinical cases · 2022Article
- Novel computer aided diagnostic models on multimodality medical images to differentiate well differentiated liposarcomas from lipomas approached by deep learning methods.Orphanet journal of rare diseases · 2022Article
- A scoring system combining clinical, radiological, and histopathological examinations for differential diagnosis between lipoma and atypical lipomatous tumor/well-differentiated liposarcoma.Scientific reports · 2022Article
- Pediatric liposarcoma: A case series and literature review.Pediatric blood & cancer · 2021Review
- Biology and Management of Dedifferentiated Liposarcoma: State of the Art and Perspectives.Journal of clinical medicine · 2021Review
- Feasibility of Developing Radiotracers for MDM2: Synthesis and Preliminary Evaluation of anPharmaceuticals (Basel, Switzerland) · 2021Article
- MDM2 Amplified Sarcomas: A Literature Review.Diagnostics (Basel, Switzerland) · 2021Review
- Accuracy of histological grades from intraoperative frozen-section diagnoses of soft-tissue tumors.International journal of clinical oncology · 2020Article
- The Utility of MDM2 and CDK4 Immunohistochemistry and MDM2 FISH in Craniofacial Osteosarcoma.Head and neck pathology · 2020Article
- Semi-comprehensive analysis of gene amplification in thymic malignant tumors using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.International journal of clinical and experimental pathology · 2020Article
- Radiomics approach to distinguish between well differentiated liposarcomas and lipomas on MRI.The British journal of surgery · 2019Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The atypical lipomatous tumor (ALT)/well-differentiated liposarcoma (WDLS) and the de-differentiated liposarcoma (DDLS) represent the most common category of liposarcomas. ALT/WDLSs and DDLSs are often difficult to distinguish from other tumors with similar morphological characteristics. In this study, we investigated whether the detection of amplified or overexpressed murine double-minute 2 (MDM2) can be a useful diagnostic ancillary aid. We used fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) to detect MDM2 amplification and protein overexpression, respectively, in 49 WDLSs, 5 DDLSs, 23 myxoid liposarcomas, 25 benign lipomatous tumors, and 75 spindle and pleomorphic sarcomas. MDM2 amplification was detected in 48 of 49 WDLSs, 5 of 5 DDLSs, 2 of 9 malignant peripheral nerve sheath tumors, and 2 of 10 myxofibrosarcomas. We did not detect MDM2 amplification in any of the benign lipomatous tumors. FISH-mediated detection of MDM2 amplification was the most valuable diagnostic aid for ALT/WDLS, as determined by using the Fisher exact test to compare two different diagnoses of 19 biopsies. On the contrary, unequivocal nuclear overexpression of MDM2 was found in only 10 of 50 ALT/WDLSs. The sensitivity and specificity of MDM2 amplification in distinguishing a DDLS from spindle and pleomorphic sarcomas were 100% and 95%, respectively, while those of MDM2 overexpression were 100% and 87%, respectively. In conclusion, our results indicate that FISH-mediated detection of MDM2 amplification is the most useful adjunct in the diagnosis of both ALT/WDLS and DDLS. However, IHC-mediated detection of MDM2 protein is useful only for the diagnosis of DDLS.
Indexed as
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23826411PMC3693195W2408106390What OpenQuestion holds
Registered trials
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