ArticlePloS one2013
Inducible Sterilization of Zebrafish by Disruption of Primordial Germ Cell Migration.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Zebrafish (Biology · 2025Article
- Temperature and photoperiod stress in zebrafish larvae: impacts on development, gene regulation and PGC migration.Fish physiology and biochemistry · 2025Article
- Application of Repetitive Sequences in Fish Cell Depletion as a Target for the CRISPR/Cas9 System.Marine biotechnology (New York, N.Y.) · 2024Article
- Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells.Frontiers in genome editing · 2024Article
- Production of all-male non-transgenic zebrafish by conditional primordial germ cell ablation.Fish physiology and biochemistry · 2023Article
- Infertility control of transgenic fluorescent zebrafish with targeted mutagenesis of theFrontiers in genetics · 2023Article
- A Germline-Specific Regulator of Mitochondrial Fusion is Required for Maintenance and Differentiation of Germline Stem and Progenitor Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Tetraspanin Cd9b plays a role in fertility in zebrafish.PloS one · 2022Article
- Fish reproductive biology - Reflecting on five decades of fundamental and translational research.General and comparative endocrinology · 2021Review
- Hcfc1a regulates neural precursor proliferation and asxl1 expression in the developing brain.BMC neuroscience · 2020Article
- Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus, by Knockdown of Primordial Germ Cell Genes with Copper-Sensitive Constructs.Marine biotechnology (New York, N.Y.) · 2018Article
- Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development.Scientific reports · 2018Article
- Expression of Thiaminase in Zebrafish (Danio rerio) is Lethal and Has Implications for Use as a Biocontainment Strategy in Aquaculture and Invasive Species.Marine biotechnology (New York, N.Y.) · 2017Article
- A pilgrim's progress: Seeking meaning in primordial germ cell migration.Stem cell research · 2017Review
- Salt Sensitive Tet-Off-Like Systems to Knockdown Primordial Germ Cell Genes for Repressible Transgenic Sterilization in Channel Catfish, Ictalurus punctatus.Marine drugs · 2017Article
- Finding their way: themes in germ cell migration.Current opinion in cell biology · 2016Review
- Production of reproductively sterile fish by a non-transgenic gene silencing technology.Scientific reports · 2015Article
- A controllable on-off strategy for the reproductive containment of fish.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During zebrafish development, a gradient of stromal-derived factor 1a (Sdf1a) provides the directional cue that guides the migration of the primordial germ cells (PGCs) to the gonadal tissue. Here we describe a method to produce large numbers of infertile fish by inducing ubiquitous expression of Sdf1a in zebrafish embryos resulting in disruption of the normal PGC migration pattern. A transgenic line of zebrafish, Tg(hsp70:sdf1a-nanos3, EGFP), was generated that expresses Sdf1a under the control of the heat-shock protein 70 (hsp70) promoter and nanos3 3?UTR. To better visualize the PGCs, the Tg(hsp70:sdf1a-nanos3, EGFP) fish were crossed with another transgenic line, Tg(kop:DsRed-nanos3), that expresses DsRed driven by the PGC-specific kop promoter. Heat treatment of the transgenic embryos caused an induction of Sdf1a expression throughout the embryo resulting in the disruption of their normal migration. Optimal embryo survival and disruption of PGC migration was achieved when transgenic embryos at the 4- to 8-cell stage were incubated at 34.5°C for 18 hours. Under these conditions, disruption of PGC migration was observed in 100% of the embryos. Sixty-four adult fish were developed from three separate batches of heat-treated embryos and all were found to be infertile males. When each male was paired with a wild-type female, only unfertilized eggs were produced and histological examination revealed that each of the adult male fish possessed severely under-developed gonads that lacked gametes. The results demonstrate that inducible Sdf1a expression is an efficient and reliable strategy to produce infertile fish. This approach makes it convenient to generate large numbers of infertile adult fish while also providing the capability to maintain a fertile brood stock.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.