ArticlePloS one2013
Putative Structural and Functional Coupling of the Mitochondrial BKCa Channel to the Respiratory Chain.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 112 citations in OpenAlex.
- Identification of a human mitochondrial isoform of TRPV1: a thermostat regulating mitochondrial maximal temperature?Cellular and molecular life sciences : CMLS · 2026Article
- Potential therapeutic targeting of BKMolecular oncology · 2026Review
- Mitochondrial potassium channels: mitochondria-specific mechanism of regulation.Biophysical reviews · 2026Review
- Application of Modulators of CaInternational journal of molecular sciences · 2025Article
- Editorial:Acta biochimica Polonica · 2025Article
- mitoBKeLife · 2024Article
- Redox Regulation of Mitochondrial Potassium Channels Activity.Antioxidants (Basel, Switzerland) · 2024Review
- Understanding mitochondrial potassium channels: 33 years after discovery.Acta biochimica Polonica · 2024Review
- Loss of the large conductance calcium-activated potassium channel causes an increase in mitochondrial reactive oxygen species in glioblastoma cells.Pflugers Archiv : European journal of physiology · 2023Article
- Effect of Quercetin on mitoBKInternational journal of molecular sciences · 2022Article
- External Hemin as an Inhibitor of Mitochondrial Large-Conductance Calcium-Activated Potassium Channel Activity.International journal of molecular sciences · 2022Article
- Luteolin-Induced Activation of Mitochondrial BKAntioxidants (Basel, Switzerland) · 2022Article
- To what extent naringenin binding and membrane depolarization shape mitoBK channel gating-A machine learning approach.PLoS computational biology · 2022Article
- Methods of Measuring Mitochondrial Potassium Channels: A Critical Assessment.International journal of molecular sciences · 2022Review
- Mitochondrial potassium channels: A novel calcitriol target.Cellular & molecular biology letters · 2022Article
- Current Challenges of Mitochondrial Potassium Channel Research.Frontiers in physiology · 2022Review
- Mitochondrial ion channels in cardiac function.American journal of physiology. Cell physiology · 2021Review
- Routes for Potassium Ions across Mitochondrial Membranes: A Biophysical Point of View with Special Focus on the ATP-Sensitive KBiomolecules · 2021Review
- Review
- Identification of the Large-Conductance CaMolecules (Basel, Switzerland) · 2021Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potassium channels have been found in the inner mitochondrial membranes of various cells. These channels regulate the mitochondrial membrane potential, the matrix volume and respiration. The activation of these channels is cytoprotective. In our study, the single-channel activity of a large-conductance Ca(2+)-regulated potassium channel (mitoBKCa channel) was measured by patch-clamping mitoplasts isolated from the human astrocytoma (glioblastoma) U-87 MG cell line. A potassium-selective current was recorded with a mean conductance of 290 pS in symmetrical 150 mM KCl solution. The channel was activated by Ca(2+) at micromolar concentrations and by the potassium channel opener NS1619. The channel was inhibited by paxilline and iberiotoxin, known inhibitors of BKCa channels. Western blot analysis, immuno-gold electron microscopy, high-resolution immunofluorescence assays and polymerase chain reaction demonstrated the presence of the BKCa channel β4 subunit in the inner mitochondrial membrane of the human astrocytoma cells. We showed that substrates of the respiratory chain, such as NADH, succinate, and glutamate/malate, decrease the activity of the channel at positive voltages. This effect was abolished by rotenone, antimycin and cyanide, inhibitors of the respiratory chain. The putative interaction of the β4 subunit of mitoBKCa with cytochrome c oxidase was demonstrated using blue native electrophoresis. Our findings indicate possible structural and functional coupling of the mitoBKCa channel with the mitochondrial respiratory chain in human astrocytoma U-87 MG cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.