ArticlePloS one2013
Determination of Serotonin and Dopamine Metabolites in Human Brain Microdialysis and Cerebrospinal Fluid Samples by UPLC-MS/MS: Discovery of Intact Glucuronide and Sulfate Conjugates.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 68 citations in OpenAlex.
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- Plant-based dietary biomarkers: from discovery to validation using mass spectrometry-based metabolomics.Frontiers in nutrition · 2026Review
- Nuclear receptor-neurotransmitter coupling links behavior to metabolic state.bioRxiv : the preprint server for biology · 2025Article
- Bile duct ligation elevates 5-HT levels in cerebral cortex of rats partly due to impairment of brain UGT1A6 expression and activity via ammonia accumulation.Redox biology · 2024Article
- Dopamine, Immunity, and Disease.Pharmacological reviews · 2023Review
- Inhaled glucocorticoid-induced metabolome changes in asthma.European journal of endocrinology · 2022Article
- Sulfation modification of dopamine in brain regulates aggregative behavior of animals.National science review · 2022Article
- Alterations of Cytochrome P450s and UDP-Glucuronosyltransferases in Brain Under Diseases and Their Clinical Significances.Frontiers in pharmacology · 2021Review
- Serotonin pathway in carcinoid syndrome: Clinical, diagnostic, prognostic and therapeutic implications.Reviews in endocrine & metabolic disorders · 2020Review
- Evaluation of electrochemical methods for tonic dopamine detectionTrends in analytical chemistry : TRAC · 2020Article
- Where Is Dopamine and how do Immune Cells See it?: Dopamine-Mediated Immune Cell Function in Health and Disease.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2020Review
- The Human UGT2B7 Nanodisc.Drug metabolism and disposition: the biological fate of chemicals · 2020Article
- Peripheral cathepsin L inhibition induces fat loss in C. elegans and mice through promoting central serotonin synthesis.BMC biology · 2019Article
- Leveraging the power of mass spectrometry to unravel complex brain pathologies.Clinical mass spectrometry (Del Mar, Calif.) · 2019Article
- Allosteres to regulate neurotransmitter sulfonation.The Journal of biological chemistry · 2019Article
- Dopamine levels in human tear fluid.Indian journal of ophthalmology · 2019Observational
- Tetrahydrobiopterin regulates monoamine neurotransmitter sulfonation.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Utility of CSF for the Diagnosis of Primary and Secondary Monoamine Neurotransmitter Deficiencies.EJIFCC · 2017Article
- Metabolomic Profiling of Post-Mortem Brain Reveals Changes in Amino Acid and Glucose Metabolism in Mental Illness Compared with Controls.Computational and structural biotechnology journal · 2016Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An UPLC-MS/MS method was developed for the determination of serotonin (5-HT), dopamine (DA), their phase I metabolites 5-HIAA, DOPAC and HVA, and their sulfate and glucuronide conjugates in human brain microdialysis samples obtained from two patients with acute brain injuries, ventricular cerebrospinal fluid (CSF) samples obtained from four patients with obstructive hydrocephalus, and a lumbar CSF sample pooled mainly from patients undergoing spinal anesthesia in preparation for orthopedic surgery. The method was validated by determining the limits of detection and quantification, linearity, repeatability and specificity. The direct method enabled the analysis of the intact phase II metabolites of 5-HT and DA, without hydrolysis of the conjugates. The method also enabled the analysis of the regioisomers of the conjugates, and several intact glucuronide and sulfate conjugates were identified and quantified for the first time in the human brain microdialysis and CSF samples. We were able to show the presence of 5-HIAA sulfate, and that dopamine-3-O-sulfate predominates over dopamine-4-O-sulfate in the human brain. The quantitative results suggest that sulfonation is a more important phase II metabolism pathway than glucuronidation in the human brain.
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