ArticlePloS one2013
A tobacco etch virus protease with increased substrate tolerance at the P1' position.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Engineering the Tobacco Etch Virus Protease toward a Platform for Traceless Cleavage Using Distal Site Prediction and Smart Library Design.ACS synthetic biology · 2025Article
- Optogenetics with Atomic Precision─A Comprehensive Review of Optical Control of Protein Function through Genetic Code Expansion.Chemical reviews · 2025Review
- Article
- Structural study for substrate recognition of human N-terminal glutamine amidohydrolase 1 in the arginine N-degron pathway.Protein science : a publication of the Protein Society · 2024Article
- Proteins that carry dual targeting signals can act as tethers between peroxisomes and partner organelles.PLoS biology · 2024Article
- Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli.Microbial cell factories · 2023Article
- Reprogramming the Cleavage Specificity of Botulinum Neurotoxin Serotype B1.ACS synthetic biology · 2022Article
- Making the cut with protease engineering.Cell chemical biology · 2022Review
- Improved yield, stability, and cleavage reaction of a novel tobacco etch virus protease mutant.Applied microbiology and biotechnology · 2022Review
- Development of a Bio-Layer Interferometry-Based Protease Assay Using HIV-1 Protease as a Model.Viruses · 2021Article
- Phage-assisted evolution of botulinum neurotoxin proteases with reprogrammed specificity.Science (New York, N.Y.) · 2021Article
- Multiple domains of bacterial and human Lon proteases define substrate selectivity.Emerging microbes & infections · 2018Article
- Article
- Engineering peptide ligase specificity by proteomic identification of ligation sites.Nature chemical biology · 2018Article
- Phage-assisted continuous evolution of proteases with altered substrate specificity.Nature communications · 2017Article
- Seven novel modulators of the analgesic target NaV 1.7 uncovered using a high-throughput venom-based discovery approach.British journal of pharmacology · 2015Article
- Article
- Photo-sensitive degron variants for tuning protein stability by light.BMC systems biology · 2014Article
- Optimization of the Production of Soluble Recombinant TEV Protease in TwoAvicenna journal of medical biotechnologyArticle
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Site-specific proteases are important tools for in vitro and in vivo cleavage of proteins. They are widely used for diverse applications, like protein purification, assessment of protein-protein interactions or regulation of protein localization, abundance or activity. Here, we report the development of a procedure to select protease variants with altered specificity based on the well-established Saccharomyces cerevisiae adenine auxotrophy-dependent red/white colony assay. We applied this method on the tobacco etch virus (TEV) protease to obtain a protease variant with altered substrate specificity at the P1' Position. In vivo experiments with tester substrates showed that the mutated TEV protease still efficiently recognizes the sequence ENLYFQ, but has almost lost all bias for the amino acid at the P1' Position. Thus, we generated a site-specific protease for synthetic approaches requiring in vivo generation of proteins or peptides with a specific N-terminal amino acid.
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