ArticlePloS one2013
Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 82 citations in OpenAlex.
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- DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products.Foods (Basel, Switzerland) · 2024Article
- rCRUX: A Rapid and Versatile Tool for Generating Metabarcoding Reference libraries in R.Environmental DNA (Hoboken, N.J.) · 2024Article
- rCRUX: A Rapid and Versatile Tool for Generating Metabarcoding Reference libraries in R.bioRxiv : the preprint server for biology · 2023Article
- DNA Barcoding Medicinal Plant Species from Indonesia.Plants (Basel, Switzerland) · 2022Article
- Molecular Characterization of Wild and Cultivated Strawberry (Genetics research · 2022Article
- A Concise Review ofPlants (Basel, Switzerland) · 2021Review
- Landscape analyses using eDNA metabarcoding and Earth observation predict community biodiversity in California.Ecological applications : a publication of the Ecological Society of America · 2021Article
- Physiological and biochemical responses involved in vegetative desiccation tolerance of resurrection plant3 Biotech · 2021Article
- Molecular identification and evolutionary relationships between the subspecies of Musa by DNA barcodes.BMC genomics · 2020Article
- Efficacy of DNA barcode internal transcribed spacer 2 (ITS 2) in phylogenetic study ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- ITS2: An Ideal DNA Barcode for the Arid Medicinal Plant Rhazya Stricta.Pharmaceutical medicine · 2019Article
- Molecular Authentication of the Medicinal Species ofFrontiers in plant science · 2019Article
- Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSelaginellaceae is a family of nonseed plants with special evolutionary significance. Plants of the family Selaginellaceae are similarly shaped and easily confused, complicating identification via traditional methods. This study explored, for the first time, the use of the DNA barcode ITS2 to identify medicinal plants of the Selaginellaceae family. METHODOLOGY/PRINCIPAL
findingsIn our study, 103 samples were collected from the main distribution areas in China; these samples represented 34 species and contained almost all of the medicinal plants of Selaginellaceae. The ITS2 region of the genome was amplified from these samples and sequenced using universal primers and reaction conditions. The success rates of the PCR amplification and sequencing were 100%. There was significant divergence between the interspecific and intraspecific genetic distances of the ITS2 regions, while the presence of a barcoding gap was obvious. Using the BLAST1 and nearest distance methods, our results proved that the ITS2 regions could successfully identify the species of all Selaginellaceae samples examined. In addition, the secondary structures of ITS2 in the helical regions displayed clear differences in stem loop number, size, position, and screw angle among the medicinal plants of Selaginellaceae. Furthermore, cluster analysis using the ITS2 barcode supported the relationship between the species of Selaginellaceae established by traditional morphological methods.
conclusionThe ITS2 barcode can effectively identify medicinal plants of Selaginellaceae. The results provide a scientific basis for the precise identification of plants of the family Selaginellaceae and the reasonable development of these resources. This study may broaden the application of DNA barcoding in the medicinal plant field and benefit phylogenetic investigations.
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