ArticlePloS one2013
Sensitivity of edge detection methods for quantifying cell migration assays.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Measurement of tissue viscosity to relate force and motion in collective cell migration.Soft matter · 2026Article
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- Design, synthesis and anti-cancer activity of novel 1,2,3-triazole hybrids of erlotinib against cervical cancer via MAPK signaling pathway.Scientific reports · 2025Article
- hiPSC-derived cardiac fibroblasts dynamically enhance the mechanical function of hiPSC-derived cardiomyocytes on an engineered substrate.Frontiers in bioengineering and biotechnology · 2025Article
- Influence of Remodeled ECM and Co-culture with iPSC-Derived Cardiac Fibroblasts on the Mechanical Function of Micropatterned iPSC-Derived Cardiomyocytes.Cardiovascular engineering and technology · 2024Article
- An engineered in vitro model of the human myotendinous junction.Acta biomaterialia · 2024Article
- Parameter identifiability and model selection for partial differential equation models of cell invasion.Journal of the Royal Society, Interface · 2024Article
- Effect of rapamycin treatment in human seminoma TCam-2 cells through inhibition of G1-S transition.Naunyn-Schmiedeberg's archives of pharmacology · 2023Article
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- cMyBP-C ablation in human engineered cardiac tissue causes progressive Ca2+-handling abnormalities.The Journal of general physiology · 2023Article
- Methanolic neem (Azadirachta indica) stem bark extract induces cell cycle arrest, apoptosis and inhibits the migration of cervical cancer cells in vitro.BMC complementary medicine and therapies · 2022Article
- A stochastic mathematical model of 4D tumour spheroids with real-time fluorescent cell cycle labelling.Journal of the Royal Society, Interface · 2022Article
- Effect of substrate stiffness on friction in collective cell migration.Scientific reports · 2022Article
- Identifying Features of Cardiac Disease Phenotypes Based on Mechanical Function in a Catecholaminergic Polymorphic Ventricular Tachycardia Model.Frontiers in bioengineering and biotechnology · 2022Article
- Spatiotemporal force and motion in collective cell migration.Scientific data · 2020Article
- Cardiovascular progenitor cells and tissue plasticity are reduced in a myocardium affected by Becker muscular dystrophy.Orphanet journal of rare diseases · 2020Article
- Disease-causing mutation in α-actinin-4 promotes podocyte detachment through maladaptation to periodic stretch.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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2 authors.
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Abstract
Quantitative imaging methods to analyze cell migration assays are not standardized. Here we present a suite of two-dimensional barrier assays describing the collective spreading of an initially-confined population of 3T3 fibroblast cells. To quantify the motility rate we apply two different automatic image detection methods to locate the position of the leading edge of the spreading population after 24, 48 and 72 hours. These results are compared with a manual edge detection method where we systematically vary the detection threshold. Our results indicate that the observed spreading rates are very sensitive to the choice of image analysis tools and we show that a standard measure of cell migration can vary by as much as 25% for the same experimental images depending on the details of the image analysis tools. Our results imply that it is very difficult, if not impossible, to meaningfully compare previously published measures of cell migration since previous results have been obtained using different image analysis techniques and the details of these techniques are not always reported. Using a mathematical model, we provide a physical interpretation of our edge detection results. The physical interpretation is important since edge detection algorithms alone do not specify any physical measure, or physical definition, of the leading edge of the spreading population. Our modeling indicates that variations in the image threshold parameter correspond to a consistent variation in the local cell density. This means that varying the threshold parameter is equivalent to varying the location of the leading edge in the range of approximately 1-5% of the maximum cell density.
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