ArticlePloS one2013
MicroRNA and piRNA profiles in normal human testis detected by next generation sequencing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 123 citations in OpenAlex.
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- Genome-Wide microRNA Expression Profiling in Human Spermatozoa and Its Relation to Sperm Quality.Genes · 2025Article
- The functions and mechanisms of piRNAs in mediating mammalian spermatogenesis and their applications in reproductive medicine.Cellular and molecular life sciences : CMLS · 2024Review
- The role of MicroRNAs as fine-tuners in the onset of puberty: a comprehensive review.Annals of pediatric endocrinology & metabolism · 2024Article
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- Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells.Molecular medicine (Cambridge, Mass.) · 2024Article
- Small RNAs, spermatogenesis, and male infertility: a decade of retrospect.Reproductive biology and endocrinology : RB&E · 2023Review
- Data-Driven Medicine in the Diagnosis and Treatment of Infertility.Journal of clinical medicine · 2022Article
- Localization and in silico-based functional analysis of miR-202 in bull testis.Reproduction in domestic animals = Zuchthygiene · 2022Article
- piRNAs and PIWI Proteins as Diagnostic and Prognostic Markers of Genitourinary Cancers.Biomolecules · 2022Review
- Transcriptome Analyses Identify Potential Key microRNAs and Their Target Genes Contributing to Ovarian Reserve.International journal of molecular sciences · 2021Article
- Seminal Plasma: Relevant for Fertility?International journal of molecular sciences · 2021Review
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- Seminal plasma miRNAs in Klinefelter syndrome and in obstructive and non-obstructive azoospermia.Molecular biology reports · 2020Article
- MiRNAs Expression Profiling of Bovine (International journal of molecular sciences · 2020Article
- piRNAs in Gastric Cancer: A New Approach Towards Translational Research.International journal of molecular sciences · 2020Review
- Expression Pattern of Seminal Plasma Extracellular Vesicle Small RNAs in Boar Semen.Frontiers in veterinary science · 2020Article
- Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri-implantation embryos.Biological reviews of the Cambridge Philosophical Society · 2019Review
- Identification of piRNAs and piRNA clusters in the testes of the Mongolian horse.Scientific reports · 2019Article
- Expressions of miR-525-3p and its target gene SEMG1 in the spermatozoa of patients with asthenozoospermia.Andrology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) are the class of small endogenous RNAs that play an important regulatory role in cells by negatively affecting gene expression at transcriptional and post-transcriptional levels. There have been extensive studies aiming to discover miRNAs and to analyze their functions in the cells from a variety of species. However, there are no published studies of miRNA profiles in human testis using next generation sequencing (NGS) technology.
resultsWe employed Solexa sequencing technology to profile miRNAs in normal human testis. Total 770 known and 5 novel human miRNAs, and 20121 piRNAs were detected, indicating that the human testis has a complex population of small RNAs. The expression of 15 known and 5 novel detected miRNAs was validated by qRT-PCR. We have also predicted the potential target genes of the abundant known and novel miRNAs, and subjected them to GO and pathway analysis, revealing the involvement of miRNAs in many important biological phenomenon including meiosis and p53-related pathways that are implicated in the regulation of spermatogenesis.
conclusionsThis study reports the first genome-wide miRNA profiles in human testis using a NGS approach. The presence of large number of miRNAs and the nature of their target genes suggested that miRNAs play important roles in spermatogenesis. Here we provide a useful resource for further elucidation of the regulatory role of miRNAs and piRNAs in the spermatogenesis. It may also facilitate the development of prophylactic strategies for male infertility.
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