ArticleMolecular vision2013
Evaluation of the percentage of ganglion cells in the ganglion cell layer of the rodent retina.
Article in Molecular vision, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed, 98 citations in OpenAlex.
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- Effects of Resveratrol on Vascular Function in Retinal Ischemia-Reperfusion Injury.Antioxidants (Basel, Switzerland) · 2023Article
- Neuroprotective effects of exogenous brain-derived neurotrophic factor on amyloid-beta 1-40-induced retinal degeneration.Neural regeneration research · 2023Article
- Colour and melanopsin mediated responses in the murine retina.Frontiers in cellular neuroscience · 2023Article
- Genetic targeting or pharmacological inhibition of galectin-3 dampens microglia reactivity and delays retinal degeneration.Journal of neuroinflammation · 2022Article
- Biological Correlations and Confounders for Quantification of Retinal Ganglion Cells by Optical Coherence Tomography Based on Studies of Outbred Mice.Translational vision science & technology · 2022Article
- Fundus imaging of retinal ganglion cells transduced by retrograde transport of rAAV2-retro.Experimental eye research · 2022Article
- Extracellular vesicles in the retina - putative roles in physiology and disease.Frontiers in molecular neuroscience · 2022Review
- Optimizing AAV2/6 microglial targeting identified enhanced efficiency in the photoreceptor degenerative environment.Molecular therapy. Methods & clinical development · 2021Article
- Magnesium acetyltaurate prevents retinal damage and visual impairment in rats through suppression of NMDA-induced upregulation of NF-κB, p53 and AP-1 (c-Jun/c-Fos).Neural regeneration research · 2021Article
- Quantification and image-derived phenotyping of retinal ganglion cell nuclei in the nee mouse model of congenital glaucoma.Experimental eye research · 2021Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeRetinal ganglion cells comprise a percentage of the neurons actually residing in the ganglion cell layer (GCL) of the rodent retina. This estimate is useful to extrapolate ganglion cell loss in models of optic nerve disease, but the values reported in the literature are highly variable depending on the methods used to obtain them.
methodsWe tested three retrograde labeling methods and two immunostaining methods to calculate ganglion cell number in the mouse retina (C57BL/6). Additionally, a double-stain retrograde staining method was used to label rats (Long-Evans). The number of total neurons was estimated using a nuclear stain and selecting for nuclei that met specific criteria. Cholinergic amacrine cells were identified using transgenic mice expressing Tomato fluorescent protein. Total neurons and total ganglion cell numbers were measured in microscopic fields of 10(4) µm(2) to determine the percentage of neurons comprising ganglion cells in each field.
resultsHistorical estimates of the percentage of ganglion cells in the mouse GCL range from 36.1% to 67.5% depending on the method used. Experimentally, retrograde labeling methods yielded a combined estimate of 50.3% in mice. A retrograde method also yielded a value of 50.21% for rat retinas. Immunolabeling estimates were higher at 64.8%. Immunolabeling may introduce overestimates, however, with non-specific labeling effects, or ectopic expression of antigens in neurons other than ganglion cells.
conclusionsSince immunolabeling methods may overestimate ganglion cell numbers, we conclude that 50%, which is consistently derived from retrograde labeling methods, is a reliable estimate of the ganglion cells in the neuronal population of the GCL.
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Identifiers
23825918PMC3695759W2119765340What OpenQuestion holds
Registered trials
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