ArticlePloS one2013
Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Molecular mechanism of hyperactivation conferred by a truncation of TRPA1.Nature communications · 2023Article
- Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins.Communications biology · 2021Article
- Peptide translocation by the lysosomal ABC transporter TAPL is regulated by coupling efficiency and activation energy.Scientific reports · 2019Article
- An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells.Biochemistry · 2018Article
- Article
- Moving the Cellular Peptidome by Transporters.Frontiers in cell and developmental biology · 2018Review
- Structural basis for perception of diverse chemical substances by T1r taste receptors.Nature communications · 2017Article
- Expression, purification, and contaminant detection for structural studies of Ralstonia metallidurance ClC protein rm1.PloS one · 2017Article
- A dual inhibition mechanism of herpesviral ICP47 arresting a conformationally thermostable TAP complex.Scientific reports · 2016Article
- Live-cell protein labelling with nanometre precision by cell squeezing.Nature communications · 2016Article
- Article
- A negative feedback modulator of antigen processing evolved from a frameshift in the cowpox virus genome.PLoS pathogens · 2014Article
- Assembly and function of the major histocompatibility complex (MHC) I peptide-loading complex are conserved across higher vertebrates.The Journal of biological chemistry · 2014Article
- An annular lipid belt is essential for allosteric coupling and viral inhibition of the antigen translocation complex TAP (transporter associated with antigen processing).The Journal of biological chemistry · 2014Article
- Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA.PloS one · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many interesting and important membrane proteins are hetero-oligomeric. However, besides naturally abundant examples, the structures of relatively few such complexes are known. Partly, this is due to difficulties in expression, stoichiometric assembly, and in the evaluation of their stability prior to crystallization trials. Here we describe a new approach, which allows rapid assessment of protein complex quality, assembly and stoichiometry, simplifying the search for conditions conducive to long-term stability and crystallization. Multicolour fluorescence size-exclusion chromatography (MC-FSEC) is used to enable tracking of individual subunits through expression, solubilization and purification steps. We show how the method has been applied to the heterodimeric transporter associated with antigen processing (TAP) and demonstrate how it may be extended in order to analyse membrane multisubunit assemblies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.