ArticlePloS one2013
Rapid detection and simultaneous genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Development of high-resolution melting (HRM) assay to differentiate the species of Shigella isolates from stool and food samples.Scientific reports · 2022Article
- Genotypic and antimicrobial resistance characterizations ofFood science & nutrition · 2020Article
- Emergence ofFrontiers in microbiology · 2020Article
- An Investigation of an Acute Gastroenteritis Outbreak:Frontiers in microbiology · 2018Article
- Trends and advances in food analysis by real-time polymerase chain reaction.Journal of food science and technology · 2016Review
- Comparative Genotypic and Phenotypic Analysis of Cronobacter Species Cultured from Four Powdered Infant Formula Production Facilities: Indication of Pathoadaptation along the Food Chain.Applied and environmental microbiology · 2015Article
- Rapid and specific detection of porcine parvovirus using real-time PCR and high resolution melting (HRM) analysis.BMC veterinary research · 2015Article
- Multiplex detection of nine food-borne pathogens by mPCR and capillary electrophoresis after using a universal pre-enrichment medium.Frontiers in microbiology · 2015Article
- Strategies for the identification and tracking of cronobacter species: an opportunistic pathogen of concern to neonatal health.Frontiers in pediatrics · 2015Review
- Postsurgical Pantoea calida meningitis: a case report.Journal of medical case reports · 2014Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cronobacter spp. is an emerging pathogen that causes meningitis, sepsis, bacteremia, and necrotizing enterocolitis in neonates and children. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis targeting the OmpA gene for the specific detection and rapid identification of Cronobacter spp. (formerly Enterobacter sakazakii) in powdered infant formula. Eleven Cronobacter field isolates and 25 reference strains were examined using one pair of primers, having the accuracy of 100% in reference to conventional methods. The assay was proved to be highly sensitive with a detection limit of 10(2) CFU/ml without pre-enrichment, and highly concordant (100%) when compared with ISO-IDF 22964 in 89 actual samples. The method performed for Cronobacter spp. detection was less than 24 h, drastically shortened, compared to several days using standard culturing method, it is probe-free and reduces a risk of PCR carryover. Moreover, all Cronobacter strains examined in this study were genotyped into two species according to their HRM profiles. The established method should provide a molecular tool for direct detection and simultaneous genotyping of Cronobacter spp. in powdered infant formula.
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