ArticlePloS one2013
Plasmodium falciparum erythrocytic stage parasites require the putative autophagy protein PfAtg7 for normal growth.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Mitophagy-mediated immune evasion: Shared strategies of pathogens.Redox biology · 2026Review
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- Exploring Quinoline Derivatives: Their Antimalarial Efficacy and Structural Features.Medicinal chemistry (Shariqah (United Arab Emirates)) · 2025Review
- Regulation of Atg8 membrane deconjugation by cysteine proteases in the malaria parasite Plasmodium berghei.Cellular and molecular life sciences : CMLS · 2023Article
- A single-cell liver atlas of Plasmodium vivax infection.Cell host & microbe · 2022Article
- Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy.Science (New York, N.Y.) · 2022Article
- Chorismate synthase mediates cerebral malaria pathogenesis by eliciting salicylic acid-dependent autophagy response in parasite.Biology open · 2020Article
- Ubiquitin-Like Modifiers: Emerging Regulators of Protozoan Parasites.Biomolecules · 2020Review
- Comparative and functional genomics of the protozoan parasite Babesia divergens highlighting the invasion and egress processes.PLoS neglected tropical diseases · 2019Article
- Plastid-endomembrane connections in apicomplexan parasites.PLoS pathogens · 2019Review
- A mutagenesis screen for essential plastid biogenesis genes in human malaria parasites.PLoS biology · 2019Article
- Characterization of the molecular mechanism of the autophagy-related Atg8-Atg3 protein interaction inThe Journal of biological chemistry · 2018Article
- Characterization of Plasmodium Atg3-Atg8 Interaction Inhibitors Identifies Novel Alternative Mechanisms of Action in Toxoplasma gondii.Antimicrobial agents and chemotherapy · 2018Article
- Article
- Basal and starvation-induced autophagy mediates parasite survival during intraerythrocytic stages ofCell death discovery · 2018Article
- Virtual Screening and Experimental Validation Identify Novel Inhibitors of the Plasmodium falciparum Atg8-Atg3 Protein-Protein Interaction.ChemMedChem · 2016Article
- Antiplasmodial activity of chloroquine analogs against chloroquine-resistant parasites, docking studies and mechanisms of drug action.Malaria journal · 2014Article
- Review
- Identification of an Atg8-Atg3 protein-protein interaction inhibitor from the medicines for Malaria Venture Malaria Box active in blood and liver stage Plasmodium falciparum parasites.Journal of medicinal chemistry · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of the Plasmodium falciparum genome reveals a limited number of putative autophagy genes, specifically the four genes involved in ATG8 lipidation, an essential step in formation of autophagosomes. In yeast, Atg8 lipidation requires the E1-type ligase Atg7, an E2-type ligase Atg3, and a cysteine protease Atg4. These four putative P. falciparum ATG (PfATG) genes are transcribed during the parasite's erythrocytic stages. PfAtg7 has relatively low identity and similarity to yeast Atg7 (14.7% and 32.2%, respectively), due primarily to long insertions typical of P. falciparum. Excluding the insertions the identity and similarity are higher (38.0% and 70.8%, respectively). This and the fact that key residues are conserved, including the catalytic cysteine and ATP binding domain, we hypothesize that PfAtg7 is the activating enzyme of PfAtg8. To assess the role of PfAtg7 we have generated two transgenic parasite lines. In one, the PfATG7 locus was modified to introduce a C-terminal hemagglutinin tag. Western blotting reveals two distinct protein species, one migrating near the predicted 150 kDa and one at approximately 65 kDa. The second transgenic line introduces an inducible degradation domain into the PfATG7 locus, allowing us to rapidly attenuate PfAtg7 protein levels. Corresponding species are also observed in this parasite line at approximately 200 kDa and 100 kDa. Upon PfATG7 attenuation parasites exhibit a slow growth phenotype indicating the essentiality of this putative enzyme for normal growth.
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