ArticlePloS one2013
A robust method to analyze copy number alterations of less than 100 kb in single cells using oligonucleotide array CGH.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Bridging the gap: a genetically validated avian chorioallantoic membrane platform for investigation of spontaneous circulating tumor cells.Molecular oncology · 2026Article
- Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Multiparametric Circulating Tumor Cell Analysis to Select Targeted Therapies for Breast Cancer Patients.Cancers · 2021Article
- GLS-driven glutamine catabolism contributes to prostate cancer radiosensitivity by regulating the redox state, stemness and ATG5-mediated autophagy.Theranostics · 2021Article
- FGF Signalling in the Self-Renewal of Colon Cancer Organoids.Scientific reports · 2019Article
- Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization.Journal of visualized experiments : JoVE · 2018Article
- Cancer Diagnosis Using a Liquid Biopsy: Challenges and Expectations.Diagnostics (Basel, Switzerland) · 2018Review
- Article
- Disseminated tumour cells with highly aberrant genomes are linked to poor prognosis in operable oesophageal adenocarcinoma.British journal of cancer · 2017Article
- Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells.Oncotarget · 2017Article
- Circulating Tumour Cell Release after Cement Augmentation of Vertebral Metastases.Scientific reports · 2017Article
- Materials and microfluidics: enabling the efficient isolation and analysis of circulating tumour cells.Chemical Society reviews · 2017Review
- Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications.BMC cancer · 2017Article
- Catch and Release: rare cell analysis from a functionalised medical wire.Scientific reports · 2017Article
- A novel microfluidic platform for size and deformability based separation and the subsequent molecular characterization of viable circulating tumor cells.International journal of cancer · 2016Article
- Non-invasive detection of genome-wide somatic copy number alterations by liquid biopsies.Molecular oncology · 2016Review
- Article
- Reliable single cell array CGH for clinical samples.PloS one · 2014Article
- Single cell genomics: advances and future perspectives.PLoS genetics · 2014Review
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Comprehensive genome wide analyses of single cells became increasingly important in cancer research, but remain to be a technically challenging task. Here, we provide a protocol for array comparative genomic hybridization (aCGH) of single cells. The protocol is based on an established adapter-linker PCR (WGAM) and allowed us to detect copy number alterations as small as 56 kb in single cells. In addition we report on factors influencing the success of single cell aCGH downstream of the amplification method, including the characteristics of the reference DNA, the labeling technique, the amount of input DNA, reamplification, the aCGH resolution, and data analysis. In comparison with two other commercially available non-linear single cell amplification methods, WGAM showed a very good performance in aCGH experiments. Finally, we demonstrate that cancer cells that were processed and identified by the CellSearch® System and that were subsequently isolated from the CellSearch® cartridge as single cells by fluorescence activated cell sorting (FACS) could be successfully analyzed using our WGAM-aCGH protocol. We believe that even in the era of next-generation sequencing, our single cell aCGH protocol will be a useful and (cost-) effective approach to study copy number alterations in single cells at resolution comparable to those reported currently for single cell digital karyotyping based on next generation sequencing data.
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