ArticlePloS one2013
Dynamics and molecular determinants of cytoplasmic lipid droplet clustering and dispersion.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Characterization of a novel variant in the HR1 domain ofF1000Research · 2021Article
- Microtubule-dependent and independent roles of spastin in lipid droplet dispersion and biogenesis.Life science alliance · 2020Article
- Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Alcohol-induced microtubule acetylation leads to the accumulation of large, immobile lipid droplets.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Article
- PEDF regulates plasticity of a novel lipid-MTOC axis in prostate cancer-associated fibroblasts.Journal of cell science · 2018Article
- Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes.Scientific reports · 2017Article
- Lipid droplet functions beyond energy storage.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine.Microbiome · 2017Article
- Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage.Nature communications · 2017Article
- Article
- Factor XIII-A transglutaminase deficient mice show signs of metabolically healthy obesity on high fat diet.Scientific reports · 2016Article
- Xanthine oxidoreductase mediates membrane docking of milk-fat droplets but is not essential for apocrine lipid secretion.The Journal of physiology · 2016Article
- Human 'brite/beige' adipocytes develop from capillary networks, and their implantation improves metabolic homeostasis in mice.Nature medicine · 2016Article
- The Altered Hepatic Tubulin Code in Alcoholic Liver Disease.Biomolecules · 2015Review
- As the fat flies: The dynamic lipid droplets of Drosophila embryos.Biochimica et biophysica acta · 2015Review
- The cell biology of fat expansion.The Journal of cell biology · 2015Review
- GRAF1a is a brain-specific protein that promotes lipid droplet clustering and growth, and is enriched at lipid droplet junctions.Journal of cell science · 2014Article
- Lipid transport in the lactating mammary gland.Journal of mammary gland biology and neoplasia · 2014Review
- Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells.Plant signaling & behavior · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Perilipin-1 (Plin1), a prominent cytoplasmic lipid droplet (CLD) binding phosphoprotein and key physiological regulator of triglyceride storage and lipolysis in adipocytes, is thought to regulate the fragmentation and dispersion of CLD that occurs in response to β-adrenergic activation of adenylate cyclase. Here we investigate the dynamics and molecular determinants of these processes using cell lines stably expressing recombinant forms of Plin1 and/or other members of the perilipin family. Plin1 and a C-terminal CLD-binding fragment of Plin1 (Plin1CT) induced formation of single dense CLD clusters near the microtubule organizing center, whereas neither an N-terminal CLD-binding fragment of Plin1, nor Plin2 or Plin3 induced clustering. Clustered CLD coated by Plin1, or Plin1CT, dispersed in response to isoproterenol, or other agents that activate adenylate cyclase, in a process inhibited by the protein kinase A inhibitor, H89, and blocked by microtubule disruption. Isoproterenol-stimulated phosphorylation of CLD-associated Plin1 on serine 492 preceded their dispersion, and live cell imaging showed that cluster dispersion involved initial fragmentation of tight clusters into multiple smaller clusters, which then fragmented into well-dispersed individual CLD. siRNA knockdown of the cortical actin binding protein, moesin, induced disaggregation of tight clusters into multiple smaller clusters, and inhibited the reaggregation of dispersed CLD into tight clusters. Together these data suggest that the clustering and dispersion processes involve a complex orchestration of phosphorylation-dependent, microtubule-dependent and independent, and microfilament dependent steps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.