ArticlePloS one2013
"Salvage microbiology": detection of bacteria directly from clinical specimens following initiation of antimicrobial treatment.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Metagenomic next-generation sequencing shotgun for the diagnosis of infection in connective tissue diseases: A retrospective study.Frontiers in cellular and infection microbiology · 2022Article
- Development of a Multiplex PCR Platform for the Rapid Detection of Bacteria, Antibiotic Resistance, andFrontiers in cellular and infection microbiology · 2019Article
- New Molecular Diagnostic Approaches to Bacterial Infections and Antibacterial Resistance.Annual review of medicine · 2018Review
- The effect of empiric antimicrobial treatment duration on detection of bacterial DNA in sterile surgical specimens.PloS one · 2017Article
- Rapid Diagnosis of Infection in the Critically Ill, a Multicenter Study of Molecular Detection in Bloodstream Infections, Pneumonia, and Sterile Site Infections.Critical care medicine · 2015Observational
- Sterile and microbial-associated intra-amniotic inflammation in preterm prelabor rupture of membranes.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015Article
- A Case of Bordetella brochiseptica at a Military Medical Facility in Hawai'i: Phenotypic and Molecular Testing of an Uncommon Human Pathogen.Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health · 2015Article
- Sterile intra-amniotic inflammation in asymptomatic patients with a sonographic short cervix: prevalence and clinical significance.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015Article
- Enterovirus D68-associated acute respiratory distress syndrome in adult, United States, 2014.Emerging infectious diseases · 2015Article
- Salvage microbiology: opportunities and challenges in the detection of bacterial pathogens following initiation of antimicrobial treatment.Expert review of molecular diagnostics · 2015Review
- Identification of occult Fusobacterium nucleatum central nervous system infection by use of PCR-electrospray ionization mass spectrometry.Journal of clinical microbiology · 2014Article
- Improved sensitivity for molecular detection of bacterial and Candida infections in blood.Journal of clinical microbiology · 2014Article
- Ureaplasma parvum prosthetic joint infection detected by PCR.Journal of clinical microbiology · 2014Article
- Direct detection of indirect transmission of Streptobacillus moniliformis rat bite fever infection.Journal of clinical microbiology · 2014Article
- A novel molecular microbiologic technique for the rapid diagnosis of microbial invasion of the amniotic cavity and intra-amniotic infection in preterm labor with intact membranes.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- Culture negative empyema in a critically ill child: an opportunity for rapid molecular diagnostics.BMC anesthesiology · 2014Article
- Improving the diagnosis of bloodstream infections: PCR coupled with mass spectrometry.BioMed research international · 2014Review
- Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundPCR coupled with electrospray ionization mass spectrometry (ESI-MS) is a diagnostic approach that has demonstrated the capacity to detect pathogenic organisms from culture negative clinical samples after antibiotic treatment has been initiated. [1] We describe the application of PCR/ESI-MS for detection of bacteria in original patient specimens that were obtained after administration of antibiotic treatment in an open investigation analysis.
methodsWe prospectively identified cases of suspected bacterial infection in which cultures were not obtained until after the initiation of antimicrobial treatment. PCR/ESI-MS was performed on 76 clinical specimens that were submitted for conventional microbiology testing from 47 patients receiving antimicrobial treatment.
findingsIn our series, 72% (55/76) of cultures obtained following initiation of antimicrobial treatment were non-diagnostic (45 negative cultures; and 10 respiratory specimens with normal flora (5), yeast (4), or coagulase-negative staphylococcus (1)). PCR/ESR-MS detected organisms in 83% (39/47) of cases and 76% (58/76) of the specimens. Bacterial pathogens were detected by PCR/ESI-MS in 60% (27/45) of the specimens in which cultures were negative. Notably, in two cases of relapse of prosthetic knee infections in patients on chronic suppressive antibiotics, the previous organism was not recovered in tissue cultures taken during extraction of the infected knee prostheses, but was detected by PCR/ESI-MS.
conclusionMolecular methods that rely on nucleic acid amplification may offer a unique advantage in the detection of pathogens collected after initiation of antimicrobial treatment and may provide an opportunity to target antimicrobial therapy and "salvage" both individual treatment regimens as well as, in select cases, institutional antimicrobial stewardship efforts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.