ArticleJournal of Zhejiang University. Science. B2013
Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China.
Article in Journal of Zhejiang University. Science. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Article
- Article
- Major biotic stresses affecting maize production in Kenya and their implications for food security.PeerJ · 2023Review
- Portable rapid detection of maize chlorotic mottle virus using RT-RAA/CRISPR-Cas12a based lateral flow assay.Frontiers in plant science · 2023Article
- Proteomic Changes during MCMV Infection Revealed by iTRAQ Quantitative Proteomic Analysis in Maize.International journal of molecular sciences · 2019Article
- Maize lethal necrosis and the molecular basis of variability in concentrations of the causal viruses in co-infected maize plant.Journal of general and molecular virology · 2019Review
- Further characterization of Maize chlorotic mottle virus and its synergistic interaction with Sugarcane mosaic virus in maize.Scientific reports · 2017Article
- Article
- Monoclonal antibody-based serological methods for detecting Citrus tristeza virus in citrus groves.Virologica Sinica · 2016Article
- Monoclonal antibody-based serological assays for detection of Potato virus S in potato plants.Journal of Zhejiang University. Science. BArticle
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327680, 1:64000, and 1:3276800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.
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