ArticlePloS one2013
Effect of Cell Shape and Dimensionality on Spindle Orientation and Mitotic Timing.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed.
- Micropatterned neural induction with heat-inactivated extracellular matrix protein by on-demand high-speed laser.Regenerative therapy · 2026Article
- The Floor-Ceiling-Chip, or 2 × 2D = Pseudo-3D-Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell-Adhesive Protein Micropatterns.Advanced healthcare materials · 2026Article
- A fine balance among key biophysical factors is required for recovery of bipolar mitotic spindle from monopolar and multipolar abnormalities.Molecular biology of the cell · 2023Article
- E-cadherin in developing murine T cells controls spindle alignment and progression through β-selection.Science advances · 2023Article
- Kv10.1 Regulates Microtubule Dynamics during Mitosis.Cancers · 2020Article
- BRCA1 controls the cell division axis and governs ploidy and phenotype in human mammary cells.Oncotarget · 2017Article
- Rolled-up functionalized nanomembranes as three-dimensional cavities for single cell studies.Nano letters · 2014Article
- Miniaturized pre-clinical cancer models as research and diagnostic tools.Advanced drug delivery reviews · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation and orientation of the mitotic spindle is a critical feature of mitosis. The morphology of the cell and the spatial distribution and composition of the cells' adhesive microenvironment all contribute to dictate the position of the spindle. However, the impact of the dimensionality of the cells' microenvironment has rarely been studied. In this study we present the use of a microwell platform, where the internal surfaces of the individual wells are coated with fibronectin, enabling the three-dimensional presentation of adhesive ligands to single cells cultured within the microwells. This platform was used to assess the effect of dimensionality and cell shape in a controlled microenvironment. Single HeLa cells cultured in circular microwells exhibited greater tilting of the mitotic spindle, in comparison to cells cultured in square microwells. This correlated with an increase in the time required to align the chromosomes at the metaphase plate due to prolonged activation of the spindle checkpoint in an actin dependent process. The comparison to 2D square patterns revealed that the dimensionality of cell adhesions alone affected both mitotic timings and spindle orientation; in particular the role of actin varied according to the dimensionality of the cells' microenvironment. Together, our data revealed that cell shape and the dimensionality of the cells' adhesive environment impacted on both the orientation of the mitotic spindle and progression through mitosis.
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Registered trials
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