ArticleChemphyschem : a European journal of chemical physics and physical chemistry2013
Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Omega-3 and -6 Fatty Acids Alter the Membrane Lipid Composition and Vesicle Size to Regulate Exocytosis and Storage of Catecholamines.ACS chemical neuroscience · 2024Article
- Anionic Species Regulate Chemical Storage in Nanometer Vesicles and Amperometrically Detected Exocytotic Dynamics.Journal of the American Chemical Society · 2022Article
- Concentration of stimulant regulates initial exocytotic molecular plasticity at single cells.Chemical science · 2022Article
- Vesicular release dynamics are altered by the interaction between the chemical cargo and vesicle membrane lipids.Chemical science · 2021Article
- Counteranions in the Stimulation Solution Alter the Dynamics of Exocytosis Consistent with the Hofmeister Series.Journal of the American Chemical Society · 2020Article
- Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells.Biophysical journal · 2020Article
- Carbon-Fiber Nanoelectrodes for Real-Time Discrimination of Vesicle Cargo in the Native Cellular Environment.ACS nano · 2020Article
- Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Mechanistic study of PpIX accumulation using the JFCR39 cell panel revealed a role for dynamin 2-mediated exocytosis.Scientific reports · 2019Article
- Electrochemistry at the Synapse.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- How does the stimulus define exocytosis in adrenal chromaffin cells?Pflugers Archiv : European journal of physiology · 2018Review
- Chemistry in a vesicle.The Journal of general physiology · 2017Article
- Fusion pores and their control of neurotransmitter and hormone release.The Journal of general physiology · 2017Review
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System.Frontiers in molecular neuroscience · 2017Article
- 'Full fusion' is not ineluctable during vesicular exocytosis of neurotransmitters by endocrine cells.Proceedings. Mathematical, physical, and engineering sciences · 2017Article
- Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2016Article
- Small molecules demonstrate the role of dynamin as a bi-directional regulator of the exocytosis fusion pore and vesicle release.Molecular psychiatry · 2015Article
- Synaptic neuropeptide release by dynamin-dependent partial release from circulating vesicles.Molecular biology of the cell · 2015Article
- Reovirus FAST Proteins Drive Pore Formation and Syncytiogenesis Using a Novel Helix-Loop-Helix Fusion-Inducing Lipid Packing Sensor.PLoS pathogens · 2015Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Dynamin is a GTPase mechanochemical enzyme involved in the late steps of endocytosis, where it separates the endocytotic vesicle from the cell membrane. However, recent reports have emphasized its role in exocytosis. In this case, dynamin may contribute to the control of the exocytotic pore, thus suggesting a direct control on the efflux of neurotransmitters. Dynasore, a selective inhibitor of the GTPase activity of dynamin, was used to investigate the role of dynamin in exocytosis. Exocytosis was analyzed by amperometry, thus revealing that dynasore inhibits exocytosis in a dose-dependent manner. Analysis of the exocytotic peaks shows that the inhibition of the GTPase activity of dynamin leads to shorter, smaller events. This observation, together with the rapid effect of dynasore, suggests that the blocking of the GTPase induces the formation of a more narrow and short-lived fusion pore. These results suggest that the GTPase properties of dynamin are involved in the duration and kinetics of exocytotic release. Interestingly, and in strong contrast with its role in endocytosis, the mechanochemical properties of dynamin appear to contribute to the dilation and stability of the pore during exocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.