ArticleDevelopment (Cambridge, England)2013
Apical constriction initiates new bud formation during monopodial branching of the embryonic chicken lung.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Biophysical processes of morphogenesis in lizard lungs.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- TGFβ determines epithelial tissue spacing by regulating mesenchymal condensation.Cell reports · 2026Article
- Patterns of mitochondrial ATP predict tissue folding.Science advances · 2026Article
- TGFβ determines epithelial tissue spacing by regulating mesenchymal condensation.bioRxiv : the preprint server for biology · 2026Article
- Epithelial cell shape changes contribute to regulation of ureteric bud branching morphogenesis.The FEBS journal · 2025Article
- Biophysical mechanisms of morphogenesis in lizard lungs.bioRxiv : the preprint server for biology · 2025Article
- Fluid secretion and luminal pressure control lateral branching morphogenesis in the embryonic avian lung.Developmental biology · 2025Article
- Viscous shear is a key force in Drosophila ventral furrow morphogenesis.Development (Cambridge, England) · 2024Article
- Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures.Scientific reports · 2024Article
- Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis.International journal of molecular sciences · 2024Article
- Mesenchyme instructs growth while epithelium directs branching in the mouse mammary gland.eLife · 2024Article
- Article
- Focal sources of FGF-10 promote the buckling morphogenesis of the embryonic airway epithelium.Biology open · 2022Article
- Epithelial cells adapt to curvature induction via transient active osmotic swelling.Developmental cell · 2022Article
- The cell polarity determinant Dlg1 facilitates epithelial invagination by promoting tissue-scale mechanical coordination.Development (Cambridge, England) · 2022Article
- Dynamic changes in epithelial cell packing during tissue morphogenesis.Current biology : CB · 2021Review
- A Chemomechanical Model for Regulation of Contractility in the Embryonic Brain Tube.Journal Of Elasticity · 2021Article
- Lung branching morphogenesis is accompanied by temporal metabolic changes towards a glycolytic preference.Cell & bioscience · 2021Article
- Patient-Specific Organoid and Organ-on-a-Chip: 3D Cell-Culture Meets 3D Printing and Numerical Simulation.Advanced biology · 2021Review
- Mechanics of Development.Developmental cell · 2021Review
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Branching morphogenesis sculpts the airway epithelium of the lung into a tree-like structure to conduct air and promote gas exchange after birth. In the avian lung, a series of buds emerges from the dorsal surface of the primary bronchus via monopodial branching to form the conducting airways; anatomically, these buds are similar to those formed by domain branching in the mammalian lung. Here, we show that monopodial branching is initiated by apical constriction of the airway epithelium, and not by differential cell proliferation, using computational modeling and quantitative imaging of embryonic chicken lung explants. Both filamentous actin and phosphorylated myosin light chain were enriched at the apical surface of the airway epithelium during monopodial branching. Consistently, inhibiting actomyosin contractility prevented apical constriction and blocked branch initiation. Although cell proliferation was enhanced along the dorsal and ventral aspects of the primary bronchus, especially before branch formation, inhibiting proliferation had no effect on the initiation of branches. To test whether the physical forces from apical constriction alone are sufficient to drive the formation of new buds, we constructed a nonlinear, three-dimensional finite element model of the airway epithelium and used it to simulate apical constriction and proliferation in the primary bronchus. Our results suggest that, consistent with the experimental results, apical constriction is sufficient to drive the early stages of monopodial branching whereas cell proliferation is dispensable. We propose that initial folding of the airway epithelium is driven primarily by apical constriction during monopodial branching of the avian lung.
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