ArticlePlant reproduction2013
External application of gametophyte-specific ZmPMEI1 induces pollen tube burst in maize.
Article in Plant reproduction, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Maize Anther Development Involves Translated Open Reading Frames From 3' Untranslated Regions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- ABA-Induced Transcriptomic Dynamics inPlants (Basel, Switzerland) · 2026Article
- Interplay of different signaling mechanisms in regulating programmed cell death during plant development and in response to stresses.Protoplasma · 2026Review
- The Role of Female and Male Genes in Regulating Pollen Tube Guidance in Flowering Plants.Genes · 2024Review
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- Genome-wide identification of the pectin methylesterase inhibitor genes inFrontiers in plant science · 2022Article
- Two Carbohydrate-Based Natural Extracts StimulateFrontiers in plant science · 2021Article
- Insights into the molecular control of cross-incompatibility in Zea mays.Plant reproduction · 2020Review
- Gene-wide identification and expression analysis of the PMEI family genes in soybean (Glycine max).3 Biotech · 2020Article
- Physiological Importance of Pectin Modifying Genes During Rice Pollen Development.International journal of molecular sciences · 2020Article
- A Complex Journey: Cell Wall Remodeling, Interactions, and Integrity During Pollen Tube Growth.Frontiers in plant science · 2020Review
- Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.Scientific reports · 2019Article
- Article
- Global Transcriptional Insights of Pollen-Pistil Interactions Commencing Self-Incompatibility and Fertilization in Tea [International journal of molecular sciences · 2019Article
- Genome-Wide Identification and Characterization of Pectin Methylesterase Inhibitor Genes inInternational journal of molecular sciences · 2018Article
- Transcriptome Analysis Provides Insight into the Molecular Mechanisms UnderlyingInternational journal of molecular sciences · 2018Article
- Genome-Wide Identification, Molecular Evolution, and Expression Profiling Analysis of Pectin Methylesterase Inhibitor Genes inInternational journal of molecular sciences · 2018Article
- Pectin methylesterase inhibitor (PMEI) family can be related to male sterility in Chinese cabbage (Brassica rapa ssp. pekinensis).Molecular genetics and genomics : MGG · 2018Article
- Multilayered signaling pathways for pollen tube growth and guidance.Plant reproduction · 2018Review
- Combined Experimental and Computational Approaches Reveal Distinct pH Dependence of Pectin Methylesterase Inhibitors.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated demethylesterification of homogalacturonan, a major component of plant cell walls, by the activity of pectin methylesterases (PMEs), plays a critical role for cell wall stability and integrity. Especially fast growing plant cells such as pollen tubes secrete large amounts of PMEs toward their apoplasmic space. PME activity itself is tightly regulated by its inhibitor named as PME inhibitor and is thought to be required especially at the very pollen tube tip. We report here the identification and functional characterization of PMEI1 from maize (ZmPMEI1). We could show that the protein acts as an inhibitor of PME but not of invertases and found that its gene is strongly expressed in both gametophytes (pollen grain and embryo sac). Promoter reporter studies showed gene activity also during pollen tube growth toward and inside the transmitting tract. All embryo sac cells except the central cell displayed strong expression. Weaker signals were visible at sporophytic cells of the micropylar region. ZmPMEI1-EGFP fusion protein is transported within granules inside the tube and accumulates at the pollen tube tip as well as at sites where pollen tubes bend and/or change growth directions. The female gametophyte putatively influences pollen tube growth behavior by exposing it to ZmPMEI1. We therefore simulated this effect by applying recombinant protein at different concentrations on growing pollen tubes. ZmPMEI1 did not arrest growth, but destabilized the cell wall inducing burst. Compared with female gametophyte secreted defensin-like ZmES4, which induces burst at the very pollen tube tip, ZmPMEI1-induced burst occurs at the subapical region. These findings indicate that ZmPMEI1 secreted by the embryo sac likely destabilizes the pollen tube wall during perception and together with other proteins such as ZmES4 leads to burst and thus sperm release.
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