ArticleThe Journal of biological chemistry2013
Rapid in vitro assembly of Caulobacter crescentus FtsZ protein at pH 6.5 and 7.2.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed.
- Evidence of conformational switch in Streptococcus pneumoniae FtsZ during polymerization.Protein science : a publication of the Protein Society · 2021Article
- Assembly properties of bacterial tubulin homolog FtsZ regulated by the positive regulator protein ZipA and ZapA from Pseudomonas aeruginosa.Scientific reports · 2020Article
- Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri.MicrobiologyOpen · 2019Article
- Species- and C-terminal linker-dependent variations in the dynamic behavior of FtsZ on membranes in vitro.Molecular microbiology · 2018Article
- FzlA, an essential regulator of FtsZ filament curvature, controls constriction rate during Caulobacter division.Molecular microbiology · 2018Article
- The intrinsically disordered C-terminal linker of FtsZ regulates protofilament dynamics and superstructureThe Journal of biological chemistry · 2017Article
- A conserved coiled-coil protein pair focuses the cytokinetic Z-ring in Caulobacter crescentus.Molecular microbiology · 2017Article
- FtsZ Constriction Force - Curved Protofilaments Bending Membranes.Sub-cellular biochemistryArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
FtsZ from most bacteria assembles rapidly in vitro, reaching a steady-state plateau in 5-10 s after addition of GTP. A recent study used a novel dynamic light-scattering technique to assay the assembly of FtsZ from Caulobacter crescentus (CcFtsZ) and reported that assembly required 10 min, ∼100 times slower than for related bacteria. Previous studies had indicated normal, rapid assembly of CcFtsZ. We have reinvestigated the assembly kinetics using a mutant L72W, where assembly of subunits into protofilaments results in a significant increase in tryptophan fluorescence. We found that assembly reached a plateau in 5-10 s and showed no change in the following 10 min. This was confirmed by 90° light scattering and negative-stain electron microscopy. The very slow kinetics in the dynamic light-scattering study may be related to a refractory state induced when the FtsZ protein is stored without nucleotide, a phenomenon that we had observed in a previous study of EcFtsZ. We conclude that CcFtsZ is not an outlier, but shows rapid assembly kinetics similar to FtsZ from related bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.