ArticleScientific reports2013
High performance, LED powered, waveguide based total internal reflection microscopy.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope.Nature communications · 2025Article
- Near-wall dynamics of single cavitation bubbles imaged by total internal reflection fluorescence.Ultrasonics sonochemistry · 2025Article
- 3D printed miniature attenuated total internal reflection assisted fluorescence microscopy.Scientific reports · 2025Article
- Photonic lantern TIRF microscopy for highly efficient, uniform, artifact-free imaging.Optics express · 2024Article
- High-angle deflection of metagrating-integrated laser emission for high-contrast microscopy.Light, science & applications · 2023Article
- Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control.Biomedical optics express · 2023Article
- Femtomolar detection of SARS-CoV-2 via peptide beacons integrated on a miniaturized TIRF microscope.Science advances · 2022Article
- Live-Cell Total Internal Reflection Fluorescence (TIRF) Microscopy to Investigate Protein Internalization Dynamics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields.Nature communications · 2021Article
- Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules.Micromachines · 2021Review
- Combined total internal reflection AF spectral-imaging and Raman spectroscopy for fast assessment of surgical margins during breast cancer surgery.Biomedical optics express · 2021Article
- Single-shot, shadowless total internal reflection fluorescence microscopy via annular fiber bundle.Optics letters · 2020Article
- Direct kinetic fingerprinting and digital counting of single protein molecules.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Metasurface-based total internal reflection microscopy.Biomedical optics express · 2020Article
- Dual optical force plate for time resolved measurement of forces and pressure distributions beneath shoes and feet.Scientific reports · 2019Article
- Watch your step! A frustrated total internal reflection approach to forensic footwear imaging.Scientific reports · 2016Article
- Structure and permeability of ion-channels by integrated AFM and waveguide TIRF microscopy.Scientific reports · 2014Article
- Eliminating unwanted far-field excitation in objective-type TIRF. Part II. combined evanescent-wave excitation and supercritical-angle fluorescence detection improves optical sectioning.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Total internal reflection fluorescence (TIRF) microscopy is a rapidly expanding optical technique with excellent surface sensitivity and limited background fluorescence. Commercially available TIRF systems are either objective based that employ expensive special high numerical aperture (NA) objectives or prism based that restrict integrating other modalities of investigation for structure-function analysis. Both techniques result in uneven illumination of the field of view and require training and experience in optics. Here we describe a novel, inexpensive, LED powered, waveguide based TIRF system that could be used as an add-on module to any standard fluorescence microscope even with low NA objectives. This system requires no alignment, illuminates the entire field evenly, and allows switching between epifluorescence/TIRF/bright field modes without adjustments or objective replacements. The simple design allows integration with other imaging systems, including atomic force microscopy (AFM), for probing complex biological systems at their native nanoscale regimes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.