ArticleBiophysical journal2013
Mapping the local organization of cell membranes using excitation-polarization-resolved confocal fluorescence microscopy.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- High contrast fluorescence polarization microscopy through double tagged photoswitchable fluorescent proteins.Npj imaging · 2025Article
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions.Life science alliance · 2023Article
- Polarization effects on the fluorescence emission of zebrafish neurons using light-sheet microscopy.Biomedical optics express · 2022Article
- Defining domain-specific orientational order in the desmosomal cadherins.Biophysical journal · 2022Article
- Unraveling the geometry of complex protein organizations by polarized fluorescence imaging.Biophysical journal · 2022Article
- Fluorescence polarization modulation super-resolution imaging provides refined dynamics orientation processes in biological samples.Light, science & applications · 2022Article
- Linearly polarized excitation enhances signals from fluorescent voltage indicators.Biophysical journal · 2021Article
- Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)PInternational journal of molecular sciences · 2021Article
- Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope.Nature communications · 2021Article
- Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools.Communications biology · 2021Article
- High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes.Nature communications · 2020Article
- Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.Journal of the American Chemical Society · 2020Review
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Variable incidence angle linear dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles.Optics express · 2020Article
- Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences.Computational and structural biotechnology journal · 2020Review
- The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.The Journal of cell biology · 2019Article
- Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Polarization-resolved microscopy reveals a muscle myosin motor-independent mechanism of molecular actin ordering during sarcomere maturation.PLoS biology · 2018Article
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7 authors.
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Abstract
Fluorescence anisotropy and linear dichroism imaging have been widely used for imaging biomolecular orientational distributions in protein aggregates, fibrillar structures of cells, and cell membranes. However, these techniques do not give access to complete orientational order information in a whole image, because their use is limited to parts of the sample where the average orientation of molecules is known a priori. Fluorescence anisotropy is also highly sensitive to depolarization mechanisms such as those induced by fluorescence energy transfer. A fully excitation-polarization-resolved fluorescence microscopy imaging that relies on the use of a tunable incident polarization and a nonpolarized detection is able to circumvent these limitations. We have developed such a technique in confocal epifluorescence microscopy, giving access to new regions of study in the complex and heterogeneous molecular organization of cell membranes. Using this technique, we demonstrate morphological changes at the subdiffraction scale in labeled COS-7 cell membranes whose cytoskeleton is perturbed. Molecular orientational order is also seen to be affected by cholesterol depletion, reflecting the strong interplay between lipid-packing regions and their nearby cytoskeleton. This noninvasive optical technique can reveal local organization in cell membranes when used as a complement to existing methods such as generalized polarization.
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