Evidence map›Paper›PMID 23822732›Full record

ArticleSource code for biology and medicine2013

GenePattern flow cytometry suite.

Josef Spidlen, Aaron Barsky, Karin Breuer, Peter Carr, Marc-Danie Nazaire, Barbara Allen Hill, Yu Qian, Ted Liefeld, Michael Reich, Jill P Mesirov and 4 more

Abstract read
In one paragraph

Article in Source code for biology and medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. flowClean: Automated identification and removal of fluorescence anomalies in flow cytometry data.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016
    Article
  5. Article
  6. Article
  7. Identification and visualization of multidimensional antigen-specific T-cell populations in polychromatic cytometry data.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Josef SpidlenTerry Fox Laboratory, British Columbia Cancer Agency, Vancouver, BC, Canada. rbrinkman@bccrc.ca.
Aaron Barsky
Karin Breuer
Peter Carr
Marc-Danie Nazaire
Barbara Allen Hill
Yu Qian
Ted Liefeld
Michael Reich
Jill P Mesirov
Peter Wilkinson
Richard H Scheuermann
Rafick-Pierre Sekaly
Ryan R Brinkman

Funding

Supporting Integrative Genomic Analysis Users with GenePatternR01GM074024 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MESIROV, JILL P. · 2005 to 2019
$6.6M
Novel Concepts for the Eradication of HIVDP1DA028871 · NIDA · VGTI FLORIDA · PI SEKALY, RAFICK PIERRE · 2009 to 2013
$4.5M
The Statistical and Computational Analysis of Flow Cytometry DataR01EB008400 · NIBIB · FRED HUTCHINSON CANCER RESEARCH CENTER · PI GOTTARDO, RAPHAEL · 2008 to 2015
$2.9M
NIBIB NIH HHS R01 EB008400NIDA NIH HHS DP1 DA028871NIGMS NIH HHS R01 GM074024
6 · The paper itself

Abstract

backgroundTraditional flow cytometry data analysis is largely based on interactive and time consuming analysis of series two dimensional representations of up to 20 dimensional data. Recent technological advances have increased the amount of data generated by the technology and outpaced the development of data analysis approaches. While there are advanced tools available, including many R/BioConductor packages, these are only accessible programmatically and therefore out of reach for most experimentalists. GenePattern is a powerful genomic analysis platform with over 200 tools for analysis of gene expression, proteomics, and other data. A web-based interface provides easy access to these tools and allows the creation of automated analysis pipelines enabling reproducible research.

resultsIn order to bring advanced flow cytometry data analysis tools to experimentalists without programmatic skills, we developed the GenePattern Flow Cytometry Suite. It contains 34 open source GenePattern flow cytometry modules covering methods from basic processing of flow cytometry standard (i.e., FCS) files to advanced algorithms for automated identification of cell populations, normalization and quality assessment. Internally, these modules leverage from functionality developed in R/BioConductor. Using the GenePattern web-based interface, they can be connected to build analytical pipelines.

conclusionsGenePattern Flow Cytometry Suite brings advanced flow cytometry data analysis capabilities to users with minimal computer skills. Functionality previously available only to skilled bioinformaticians is now easily accessible from a web browser.

Identifiers

PMID23822732
PMCPMC3717030

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.