ArticleMolecular microbiology2013
Twin-arginine translocation system (tat) mutants of Salmonella are attenuated due to envelope defects, not respiratory defects.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 54 citations in OpenAlex.
- The role of cytochrome oxidases in bacterial virulence.Infection and immunity · 2026Review
- Understanding the role of pyruvate dehydrogenase inInfection and immunity · 2026Article
- Structure and function of Salmonella inner membrane.Archives of microbiology · 2026Review
- Heme in Bacterial Pathogenesis and as an Antimicrobial Target.Chemical reviews · 2025Review
- The twin-arginine translocation system is vital for cell adhesion and uptake of iron in the cystic fibrosis pathogenVirulence · 2024Article
- Metalation of Extracytoplasmic Proteins and Bacterial Cell Envelope Homeostasis.Annual review of microbiology · 2024Review
- Cytochrome oxidase requirements in Bordetella reveal insights into evolution towards life in the mammalian respiratory tract.PLoS pathogens · 2024Article
- Envelope Stress Activates Expression of the Twin Arginine Translocation (Tat) System in Salmonella.Microbiology spectrum · 2022Article
- The biogenesis of β-lactamase enzymes.Microbiology (Reading, England) · 2022Review
- Article
- The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilicFrontiers in cellular and infection microbiology · 2022Article
- Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensaleLife · 2021Article
- Bacterial Oxidases of the CytochromeAntioxidants & redox signaling · 2021Review
- Twin-arginine translocation (Tat) mutants inFEMS microbes · 2021Article
- The Tat system and its dependent cell division proteins are critical for virulence of extra-intestinal pathogenicVirulence · 2020Article
- The Twin-Arginine Translocation System Is Important for Stress Resistance and Virulence of Brucella melitensis.Infection and immunity · 2020Article
- Twin-Arginine Translocation System Is Involved in Citrobacter rodentium Fitness in the Intestinal Tract.Infection and immunity · 2020Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- The Small Protein CydX Is Required for CytochromeJournal of bacteriology · 2020Article
- Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.PLoS pathogens · 2019Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The twin-arginine translocation system (Tat) transports folded proteins across the cytoplasmic membrane and is critical to virulence in Salmonella and other pathogens. Experimental and bioinformatic data indicate that 30 proteins are exported via Tat in Salmonella Typhimurium. However, there are no data linking specific Tat substrates with virulence. We inactivated every Tat-exported protein and determined the virulence phenotype of mutant strains. Although a tat mutant is highly attenuated, no single Tat-exported substrate accounts for this virulence phenotype. Rather, the attenuation is due primarily to envelope defects caused by failure to translocate three Tat substrates, the N-acetylmuramoyl-l-alanine amidases, AmiA and AmiC, and the cell division protein, SufI. Strikingly, neither the amiA amiC nor the sufI mutations alone conferred any virulence defect. Although AmiC and SufI have previously been localized to the divisome, the synthetic phenotypes observed are the first to suggest functional overlap. Many Tat substrates are involved in anaerobic respiration, but we show that a mutant completely deficient in anaerobic respiration retains full virulence in both the oral and systemic phases of infection. Similarly, an obligately aerobic mutant is fully virulent. These results suggest that in the classic mouse model of infection, S. Typhimurium is replicating only in aerobic environments.
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