ArticleJournal of the American Chemical Society2013
Quantifying elongation rhythm during full-length protein synthesis.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Codon deoptimization of multispecific biologics reduces mispairing during transient mammalian protein expression.Frontiers in bioengineering and biotechnology · 2026Article
- Structure and sequence engineering approaches to improve in vivo expression of nucleic acid-delivered antibodies.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Strategies for Overcoming the Single-Molecule Concentration Barrier.ACS measurement science au · 2023Review
- Site-Specific Fluorescent Labeling of RNA Interior Positions.Molecules (Basel, Switzerland) · 2021Review
- Applications of Protein Engineering and Directed Evolution in Plant Research.Plant physiology · 2019Review
- Context-Specific Action of Ribosomal Antibiotics.Annual review of microbiology · 2018Review
- Codon usage clusters correlation: towards protein solubility prediction in heterologous expression systems in E. coli.Scientific reports · 2018Article
- Origins of the Mechanochemical Coupling of Peptide Bond Formation to Protein Synthesis.Journal of the American Chemical Society · 2018Article
- %MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding.Protein science : a publication of the Protein Society · 2018Article
- tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis.Biophysical journal · 2017Article
- Kinetics of CrPV and HCV IRES-mediated eukaryotic translation using single-molecule fluorescence microscopy.RNA (New York, N.Y.) · 2017Article
- A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems.Scientific reports · 2017Article
- Mutational Pressure in Zika Virus: Local ADAR-Editing Areas Associated with Pauses in Translation and Replication.Frontiers in cellular and infection microbiology · 2017Article
- Analysis of Translation Elongation Dynamics in the Context of an Escherichia coli Cell.Biophysical journal · 2016Article
- Potential for cellular stress response to hepatic factor VIII expression from AAV vector.Molecular therapy. Methods & clinical development · 2016Article
- A simple real-time assay for in vitro translation.RNA (New York, N.Y.) · 2015Article
- A critical analysis of codon optimization in human therapeutics.Trends in molecular medicine · 2014Review
- Deducing the kinetics of protein synthesis in vivo from the transition rates measured in vitro.PLoS computational biology · 2014Article
- Engine out of the chassis: cell-free protein synthesis and its uses.FEBS letters · 2014Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Pauses regulate the rhythm of ribosomal protein synthesis. Mutations disrupting even minor pauses can give rise to improperly formed proteins and human disease. Such minor pauses are difficult to characterize by ensemble methods, but can be readily examined by single-molecule (sm) approaches. Here we use smFRET to carry out real-time monitoring of the expression of a full-length protein, the green fluorescent protein variant Emerald GFP. We demonstrate significant correlations between measured elongation rates and codon and isoacceptor tRNA usage, and provide a quantitative estimate of the effect on elongation rate of replacing a codon recognizing an abundant tRNA with a synonymous codon cognate to a rarer tRNA. Our results suggest that tRNA selection plays an important general role in modulating the rates and rhythms of protein synthesis, potentially influencing simultaneous co-translational processes such as folding and chemical modification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.