ArticleG3 (Bethesda, Md.)2013
Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.
Article in G3 (Bethesda, Md.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 97 citations in OpenAlex.
- Functional diversity of the Osiris gene family in the brown planthopper.Crop health · 2025Article
- Phylogenetic Analysis of 590 Species Reveals Distinct Evolutionary Patterns of Intron-Exon Gene Structures Across Eukaryotic Lineages.Molecular biology and evolution · 2024Article
- Sex-biased gene content is associated with sex chromosome turnover in Danaini butterflies.Molecular ecology · 2024Article
- Time-course transcriptome data of silk glands in day 0-7 last-instar larvae of Bombyx mori (w1 pnd strain).Scientific data · 2024Article
- Ecdysteroid UDP-Glucosyltransferase Expression inJournal of fungi (Basel, Switzerland) · 2023Article
- Full-Length Transcriptome of the Great Himalayan Leaf-Nosed Bats (International journal of molecular sciences · 2023Article
- SilkBase: an integrated transcriptomic and genomic database for Bombyx mori and related species.Database : the journal of biological databases and curation · 2022Article
- Article
- Reference Transcriptome Data in SilkwormInsects · 2021Article
- Lepidopteran wing scales contain abundant cross-linked film-forming histidine-rich cuticular proteins.Communications biology · 2021Article
- Estrogen-Related Receptor Influences the Hemolymph Glucose Content by Regulating Midgut Trehalase Gene Expression in the Last Instar Larvae ofInternational journal of molecular sciences · 2021Article
- Host Response against Virus Infection in an Insect: Bidensovirus Infection Effect on Silkworm (Antioxidants (Basel, Switzerland) · 2021Review
- An update of KAIKObase, the silkworm genome database.Database : the journal of biological databases and curation · 2021Article
- The Response of the Estrogen-Related Receptor to 20-Hydroxyecdysone inFrontiers in physiology · 2021Article
- The Landscapes of Full-Length Transcripts and Splice Isoforms as Well as Transposons Exonization in the Lepidopteran Model System,Frontiers in genetics · 2021Article
- Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator.Insect science · 2020Article
- A transcriptome atlas of silkworm silk glands revealed by PacBio single-molecule long-read sequencing.Molecular genetics and genomics : MGG · 2020Article
- The C- and G-value paradox with polyploidy, repeatomes, introns, phenomes and cell economy.Genes & genomics · 2020Review
- Effects of Osiris9a on Silk Properties inInternational journal of molecular sciences · 2020Article
- Involvement of Two Paralogous Methoprene-Tolerant Genes in the Regulation of Vitellogenin and Vitellogenin Receptor Expression in the Rice Stem Borer,Frontiers in genetics · 2020Article
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Authors and funding
36 authors at 10 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of a complete genomic sequence of silkworm, the model species of Lepidoptera, laid a foundation for its functional genomics. A more complete annotation of the genome will benefit functional and comparative studies and accelerate extensive industrial applications for this insect. To realize these goals, we embarked upon a large-scale full-length cDNA collection from 21 full-length cDNA libraries derived from 14 tissues of the domesticated silkworm and performed full sequencing by primer walking for 11,104 full-length cDNAs. The large average intron size was 1904 bp, resulting from a high accumulation of transposons. Using gene models predicted by GLEAN and published mRNAs, we identified 16,823 gene loci on the silkworm genome assembly. Orthology analysis of 153 species, including 11 insects, revealed that among three Lepidoptera including Monarch and Heliconius butterflies, the 403 largest silkworm-specific genes were composed mainly of protective immunity, hormone-related, and characteristic structural proteins. Analysis of testis-/ovary-specific genes revealed distinctive features of sexual dimorphism, including depletion of ovary-specific genes on the Z chromosome in contrast to an enrichment of testis-specific genes. More than 40% of genes expressed in specific tissues mapped in tissue-specific chromosomal clusters. The newly obtained FL-cDNA sequences enabled us to annotate the genome of this lepidopteran model insect more accurately, enhancing genomic and functional studies of Lepidoptera and comparative analyses with other insect orders, and yielding new insights into the evolution and organization of lepidopteran-specific genes.
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