ArticleThe Journal of biological chemistry2013
Phosphatidylglycerol directs binding and inhibitory action of EIIAGlc protein on the maltose transporter.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- How Bacterial Pathogens Coordinate Appetite with Virulence.Microbiology and molecular biology reviews : MMBR · 2023Review
- The impact of metabolism on the adaptation of organisms to environmental change.Frontiers in cell and developmental biology · 2023Article
- Circularized fluorescent nanodiscs for probing protein-lipid interactions.Communications biology · 2022Article
- One-step construction of circularized nanodiscs using SpyCatcher-SpyTag.Nature communications · 2021Article
- Developing Nanodisc-ID for label-free characterizations of membrane proteins.Communications biology · 2021Article
- Cross Talk among Transporters of the Phosphoenolpyruvate-Dependent Phosphotransferase System in Bacillus subtilis.Journal of bacteriology · 2018Article
- The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution.eLife · 2018Article
- Interaction of the Antimicrobial Peptide Aurein 1.2 and Charged Lipid Bilayer.Scientific reports · 2017Article
- Negative Stain Single-particle EM of the Maltose Transporter in Nanodiscs Reveals Asymmetric Closure of MalKThe Journal of biological chemistry · 2017Article
- Regulation of CsrB/C sRNA decay by EIIA(Glc) of the phosphoenolpyruvate: carbohydrate phosphotransferase system.Molecular microbiology · 2016Article
- Sequential Action of MalE and Maltose Allows Coupling ATP Hydrolysis to Translocation in the MalFGK2 Transporter.The Journal of biological chemistry · 2015Article
- The allosteric regulatory mechanism of the Escherichia coli MetNI methionine ATP binding cassette (ABC) transporter.The Journal of biological chemistry · 2015Article
- Nucleotide-free MalK drives the transition of the maltose transporter to the inward-facing conformation.The Journal of biological chemistry · 2014Article
- Distinct effects of different phosphatidylglycerol species on mouse keratinocyte proliferation.PloS one · 2014Article
- An integrated transport mechanism of the maltose ABC importer.Research in microbiologyReview
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The signal-transducing protein EIIA(Glc) belongs to the phosphoenolpyruvate carbohydrate phosphotransferase system. In its dephosphorylated state, EIIA(Glc) is a negative regulator for several permeases, including the maltose transporter MalFGK2. How EIIA(Glc) is targeted to the membrane, how it interacts with the transporter, and how it inhibits sugar uptake remain obscure. We show here that acidic phospholipids together with the N-terminal tail of EIIA(Glc) are essential for the high affinity binding of the protein to the transporter. Using protein docking prediction and chemical cross-linking, we demonstrate that EIIA(Glc) binds to the MalK dimer, interacting with both the nucleotide-binding and the C-terminal regulatory domains. Dissection of the ATPase cycle reveals that EIIA(Glc) does not affect the binding of ATP but rather inhibits the capacity of MalK to cleave ATP. We propose a mechanism of maltose transport inhibition by this central amphitropic regulatory protein.
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