ArticleCell and tissue research2013
Intestinal stem cells remain viable after prolonged tissue storage.
Article in Cell and tissue research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Role of Focal Adhesion Kinase and NRF2 Pathways in Gastrointestinal Wound Healing.International journal of molecular sciences · 2026Review
- An Adaptable Protocol to Generate a Murine Enteroid-Macrophage Co-Culture System.International journal of molecular sciences · 2024Article
- Development potential of extracellular matrix hydrogels as hemostatic materials.Frontiers in bioengineering and biotechnology · 2023Review
- Culture of equine intestinal epithelial stem cells after delayed tissue storage for future applications.BMC veterinary research · 2022Article
- From Patient Material to New Discoveries: a Methodological Review and Guide for Intestinal Stem Cell Researchers.Stem cell reviews and reports · 2022Review
- Article
- Preservation of reserve intestinal epithelial stem cells following severe ischemic injury.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- Review
- A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture.Cellular and molecular gastroenterology and hepatology · 2018Article
- A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- GI stem cells - new insights into roles in physiology and pathophysiology.The Journal of physiology · 2016Review
- Tissue underlying the intestinal epithelium elicits proliferation of intestinal stem cells following cytotoxic damage.Cell and tissue research · 2015Article
- Establishment of human epithelial enteroids and colonoids from whole tissue and biopsy.Journal of visualized experiments : JoVE · 2015Article
- Animal models of ischemia-reperfusion-induced intestinal injury: progress and promise for translational research.American journal of physiology. Gastrointestinal and liver physiology · 2015Review
- The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures.PloS one · 2015Article
- Establishment of Gastrointestinal Epithelial Organoids.Current protocols in mouse biology · 2013Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Intestinal stem cells (ISCs) are responsible for renewal of the epithelium both during normal homeostasis and following injury. As such, they have significant therapeutic potential. However, whether ISCs can survive tissue storage is unknown. We hypothesize that, although the majority of epithelial cells might die, ISCs would remain viable for at least 24 h at 4 °C. To explore this hypothesis, jejuna of C57Bl6/J or Lgr5-LacZ mice were removed and either processed immediately or placed in phosphate-buffered saline at 4 °C. Delayed isolation of epithelium was performed after 24, 30, or 48 h storage. At the light microscope level, despite extensive apoptosis of villus epithelial cells, small intestinal crypts remained morphologically intact for 30 h and ISCs were identifiable via Lgr5-LacZ positivity. Electron microscopy showed that ISCs retained high integrity for 24 h. When assessed by flow cytometry, ISCs were more resistant to degeneration than the rest of the epithelium, including neighboring Paneth cells, with higher viability across all time points. Cultured isolated crypts showed no loss of capacity to form complex enteroids after 24 h tissue storage, with efficiencies after 7 days of culture remaining above 80 %. By 30 h storage, efficiencies declined but budding capability was retained. We conclude that, with delay in isolation, ISCs remain viable and retain their proliferative capacity. In contrast, the remainder of the epithelium, including the Paneth cells, exhibits degeneration and programmed cell death. If these findings are recapitulated in human tissue, storage at 4 °C might offer a valuable temporal window for the harvesting of crypts or ISCs for therapeutic application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.