ArticleMolecular & cellular proteomics : MCP2013
The GlycoFilter: a simple and comprehensive sample preparation platform for proteomics, N-glycomics and glycosylation site assignment.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- What Can N-glycomics and N-glycoproteomics of Cerebrospinal Fluid Tell Us about Alzheimer Disease?Biomolecules · 2021Review
- An in-depth Comparison of the Pediatric and Adult Urinary N-glycomes.Molecular & cellular proteomics : MCP · 2020Article
- Direct Analysis of NativeJournal of the American Society for Mass Spectrometry · 2020Article
- Characterizing Patients with Recurrent Urinary Tract Infections in Vesicoureteral Reflux: A Pilot Study of the Urinary Proteome.Molecular & cellular proteomics : MCP · 2020Article
- Mapping human N-linked glycoproteins and glycosylation sites using mass spectrometry.Trends in analytical chemistry : TRAC · 2019Article
- Article
- Analysis of Mammalian O-Glycopeptides-We Have Made a Good Start, but There is a Long Way to Go.Molecular & cellular proteomics : MCP · 2018Review
- Current state of the art for enhancing urine biomarker discovery.Expert review of proteomics · 2016Review
- Core-6 fucose and the oligomerization of the 1918 pandemic influenza viral neuraminidase.Biochemical and biophysical research communications · 2016Article
- Universal Solid-Phase Reversible Sample-Prep for Concurrent Proteome and N-Glycome Characterization.Journal of proteome research · 2016Article
- Review
- Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma.Molecular & cellular proteomics : MCP · 2015Article
- Dual modifications strategy to quantify neutral and sialylated N-glycans simultaneously by MALDI-MS.Analytical chemistry · 2014Article
- Absolute quantitation of glycosylation site occupancy using isotopically labeled standards and LC-MS.Journal of the American Society for Mass Spectrometry · 2014Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Current strategies to study N-glycoproteins in complex samples are often discrete, focusing on either N-glycans or N-glycosites enriched by sugar-based techniques. In this study we report a simple and rapid sample preparation platform, the GlycoFilter, which allows a comprehensive characterization of N-glycans, N-glycosites, and proteins in a single workflow. Both PNGase F catalyzed de-N-glycosylation and trypsin digestions are accelerated by microwave irradiation and performed sequentially in a single spin filter. Both N-glycans and peptides (including de-N-glycosylated peptides) are separately collected by filtration. The condition to effectively collect complex and heterogeneous N-glycans was established on model glycoproteins, bovine ribonuclease B, bovine fetuin, and human serum IgG. With this platform, the N-glycome, N-glycoproteome and proteome of human urine and plasma were characterized. Overall, a total of 865 and 295 N-glycosites were identified from three pairs of urine and plasma samples, respectively. Many sites were defined unambiguously as partially occupied by the detection of their nonsugar-modified peptides (128 from urine and 61 from plasma), demonstrating that partial occupancy of N-glycosylation occurs frequently. Given the likely high prevalence and variability of partial occupancy, glycoprotein quantification based exclusively on deglycosylated peptides may lead to inaccurate quantification.
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Registered trials
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