ArticleMethods (San Diego, Calif.)2013
Dissecting non-coding RNA mechanisms in cellulo by Single-molecule High-Resolution Localization and Counting.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- BNP-Track: a framework for superresolved tracking.Nature methods · 2024Article
- MaxCal can infer models from coupled stochastic trajectories of gene expression and cell division.Biophysical journal · 2023Article
- Tracking the Message: Applying Single Molecule Localization Microscopy to Cellular RNA Imaging.Chembiochem : a European journal of chemical biology · 2023Review
- Illuminating RNA biology through imaging.Nature cell biology · 2022Review
- Modeling Non-additive Effects in Neighboring Chemically Identical Fluorophores.The journal of physical chemistry. B · 2022Article
- RNA and liquid-liquid phase separation.Non-coding RNA research · 2021Review
- Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.Molecular cell · 2020Article
- Following the messenger: Recent innovations in live cell single molecule fluorescence imaging.Wiley interdisciplinary reviews. RNA · 2020Review
- Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.Molecular cell · 2019Article
- Coming Together: RNAs and Proteins Assemble under the Single-Molecule Fluorescence Microscope.Cold Spring Harbor perspectives in biology · 2019Review
- Maximum Caliber Can Build and Infer Models of Oscillation in a Three-Gene Feedback Network.The journal of physical chemistry. B · 2019Article
- From "Cellular" RNA to "Smart" RNA: Multiple Roles of RNA in Genome Stability and Beyond.Chemical reviews · 2018Review
- Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.Nature cell biology · 2017Article
- Building Predictive Models of Genetic Circuits Using the Principle of Maximum Caliber.Biophysical journal · 2017Article
- In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines.Protein science : a publication of the Protein Society · 2017Article
- Resolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution.Cell reports · 2017Article
- A novel method to accurately locate and count large numbers of steps by photobleaching.Molecular biology of the cell · 2016Article
- Lights, camera, action! Capturing the spliceosome and pre-mRNA splicing with single-molecule fluorescence microscopy.Wiley interdisciplinary reviews. RNA · 2016Review
- KRAS Engages AGO2 to Enhance Cellular Transformation.Cell reports · 2016Article
- Meeting report: SMART timing--principles of single molecule techniques course at the University of Michigan 2014.Biopolymers · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Non-coding RNAs (ncRNAs) recently were discovered to outnumber their protein-coding counterparts, yet their diverse functions are still poorly understood. Here we report on a method for the intracellular Single-molecule High-Resolution Localization and Counting (iSHiRLoC) of microRNAs (miRNAs), a conserved, ubiquitous class of regulatory ncRNAs that controls the expression of over 60% of all mammalian protein coding genes post-transcriptionally, by a mechanism shrouded by seemingly contradictory observations. We present protocols to execute single particle tracking (SPT) and single-molecule counting of functional microinjected, fluorophore-labeled miRNAs and thereby extract diffusion coefficients and molecular stoichiometries of micro-ribonucleoprotein (miRNP) complexes from living and fixed cells, respectively. This probing of miRNAs at the single molecule level sheds new light on the intracellular assembly/disassembly of miRNPs, thus beginning to unravel the dynamic nature of this important gene regulatory pathway and facilitating the development of a parsimonious model for their obscured mechanism of action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.