ArticlePLoS neglected tropical diseases2013
Bacillus thuringiensis-derived Cry5B has potent anthelmintic activity against Ascaris suum.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Identification of a Novel Isolate of the Poultry RoundwormVeterinary sciences · 2026Article
- Calcineurin-dependent 20E signaling pathway enhances attacin expression to prime the immunity of Helicoverpa armigera against Bacillus thuringiensis Cry1Ac toxin.PLoS pathogens · 2026Article
- Comparative Analysis of Gut Microbiomes in Parasitic Roundworms Reveals Phylogeny-Associated Community Structure and Functional Adaptation.Transboundary and emerging diseases · 2026Article
- Bacillus thuringiensis Cry14A family proteins as novel anthelmintics against gastrointestinal nematode parasites.PLoS neglected tropical diseases · 2024Article
- The nematode (Ascaris suum) intestine is a location of synergistic anthelmintic effects of Cry5B and levamisole.PLoS pathogens · 2024Article
- Comparative Analysis ofAnimals : an open access journal from MDPI · 2024Article
- Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids.Advances in parasitology · 2024Article
- Abundance, distribution, and expression of nematicidal crystal protein genes in Bacillus thuringiensis strains from diverse habitats.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Diethylcarbamazine, TRP channels and CaScientific reports · 2022Article
- Insights from the Structure of an Active Form ofToxins · 2022Article
- Efficient and Scalable Process to Produce Novel and Highly Bioactive Purified Cytosolic Crystals from Bacillus thuringiensis.Microbiology spectrum · 2022Article
- An inactivated bacterium (paraprobiotic) expressingOne health (Amsterdam, Netherlands) · 2021Article
- Yeast Particle Encapsulation of Scaffolded Terpene Compounds for Controlled Terpene Release.Foods (Basel, Switzerland) · 2021Article
- Recombinant Paraprobiotics as a New Paradigm for Treating Gastrointestinal Nematode Parasites of Humans.Antimicrobial agents and chemotherapy · 2021Article
- Combating Parasitic Nematode Infections, Newly Discovered Antinematode Compounds from Marine Epiphytic Bacteria.Microorganisms · 2020Review
- A new paraprobiotic-based treatment for control of Haemonchus contortus in sheep.International journal for parasitology. Drugs and drug resistance · 2020Article
- Cholinergic receptors on intestine cells of Ascaris suum and activation of nAChRs by levamisole.International journal for parasitology. Drugs and drug resistance · 2020Article
- Article
- Bacillus thuringiensis Cry5B protein as a new pan-hookworm cure.International journal for parasitology. Drugs and drug resistance · 2018Article
- In vivo and in vitro studies of Cry5B and nicotinic acetylcholine receptor agonist anthelmintics reveal a powerful and unique combination therapy against intestinal nematode parasites.PLoS neglected tropical diseases · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Ascaris suum and Ascaris lumbricoides are two closely related geo-helminth parasites that ubiquitously infect pigs and humans, respectively. Ascaris suum infection in pigs is considered a good model for A. lumbricoides infection in humans because of a similar biology and tissue migration to the intestines. Ascaris lumbricoides infections in children are associated with malnutrition, growth and cognitive stunting, immune defects, and, in extreme cases, life-threatening blockage of the digestive tract and aberrant migration into the bile duct and peritoneum. Similar effects can be seen with A. suum infections in pigs related to poor feed efficiency and performance. New strategies to control Ascaris infections are needed largely due to reduced treatment efficacies of current anthelmintics in the field, the threat of resistance development, and the general lack of new drug development for intestinal soil-transmitted helminths for humans and animals. Here we demonstrate for the first time that A. suum expresses the receptors for Bacillus thuringiensis crystal protein and novel anthelmintic Cry5B, which has been previously shown to intoxicate hookworms and which belongs to a class of proteins considered non-toxic to vertebrates. Cry5B is able to intoxicate A. suum larvae and adults and triggers the activation of the p38 mitogen-activated protein kinase pathway similar to that observed with other nematodes. Most importantly, two moderate doses of 20 mg/kg body weight (143 nM/kg) of Cry5B resulted in a near complete cure of intestinal A. suum infections in pigs. Taken together, these results demonstrate the excellent potential of Cry5B to treat Ascaris infections in pigs and in humans and for Cry5B to work effectively in the human gastrointestinal tract.
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