ArticlePLoS genetics2013
Evidence for two different regulatory mechanisms linking replication and segregation of vibrio cholerae chromosome II.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae.Nature communications · 2025Article
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.FEMS microbiology reviews · 2024Review
- Cell cycle-coordinated maintenance of theEcoSal Plus · 2023Review
- Dynamic ParB-DNA interactions initiate and maintain a partition condensate for bacterial chromosome segregation.Nucleic acids research · 2023Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Conjugative plasmid-encoded toxin-antitoxin system PrpT/PrpA directly controls plasmid copy number.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Characterization of ori and parS-like functions in secondary genome replicons inLife science alliance · 2021Article
- Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2.Nucleic acids research · 2020Article
- Crosstalk Regulation Between Bacterial Chromosome Replication and Chromosome Partitioning.Frontiers in microbiology · 2019Review
- Vibrio cholerae chromosome 2 copy number is controlled by the methylation-independent binding of its monomeric initiator to the chromosome 1 crtS site.Nucleic acids research · 2018Article
- Pseudomonas aeruginosa partitioning protein ParB acts as a nucleoid-associated protein binding to multiple copies of a parS-related motif.Nucleic acids research · 2018Article
- Chromosome 1 licenses chromosome 2 replication in Vibrio cholerae by doubling the crtS gene dosage.PLoS genetics · 2018Article
- Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes.Frontiers in microbiology · 2018Review
- Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli.Antibiotics (Basel, Switzerland) · 2017Article
- Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase.Nucleic acids research · 2017Article
- Random versus Cell Cycle-Regulated Replication Initiation in Bacteria: Insights from Studying Vibrio cholerae Chromosome 2.Microbiology and molecular biology reviews : MMBR · 2017Review
- Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315.PLoS genetics · 2016Article
- Optimization and Characterization of the Synthetic Secondary Chromosome synVicII inFrontiers in bioengineering and biotechnology · 2016Article
- DNA Replication Initiation Is Blocked by a Distant Chromosome-Membrane Attachment.Current biology : CB · 2015Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encoded by chromosome II of the model multichromosome bacterium, Vibrio cholerae. We find by the ChIP-chip assay that ParB2, a centromere binding protein, spreads beyond the centromere and covers a replication inhibitory site (a 39-mer). Unexpectedly, without nucleation at the centromere, ParB2 could also bind directly to a related 39-mer. The 39-mers are the strongest inhibitors of chromosome II replication and they mediate inhibition by binding the replication initiator protein. ParB2 thus appears to promote replication by out-competing initiator binding to the 39-mers using two mechanisms: spreading into one and direct binding to the other. We suggest that both these are novel mechanisms to coordinate replication initiation with segregation of chromosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.