ArticleProceedings of the National Academy of Sciences of the United States of America2013
Matrix-dependent adhesion mediates network responses to physiological stimulation of the osteocyte cell process.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone.bioRxiv : the preprint server for biology · 2026Article
- Piezo1-mediated mechanotransduction controls osteocyte maturation and dendrite development via a YAP-CCN-Src signaling axis.Nature communications · 2025Article
- Extracellular Matrix Stiffness Modulates Myopia Scleral Remodeling Through Integrin/F-Actin/YAP Axis.Investigative ophthalmology & visual science · 2025Article
- Osteocyte Sptbn1 Deficiency Alters Cell Survival and Mechanotransduction Following Formation of Plasma Membrane Disruptions (PMD) from Mechanical Loading.Calcified tissue international · 2024Article
- Article
- Mechanotransduction in subchondral bone microenvironment and targeted interventions for osteoarthritis.Mechanobiology in medicine · 2024Review
- Regulation of the integrin αVβ3- actin filaments axis in early osteogenic differentiation of human mesenchymal stem cells under cyclic tensile stress.Cell communication and signaling : CCS · 2023Article
- The bone marrow side of axial spondyloarthritis.Nature reviews. Rheumatology · 2023Review
- Osteocyte β3 integrin promotes bone mass accrual and force-induced bone formation in mice.Journal of orthopaedic translation · 2023Article
- Mapping the Response of Human Osteocytes in Native Matrix to Mechanical Loading Using RNA Sequencing.JBMR plus · 2023Article
- A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes.Journal of visualized experiments : JoVE · 2023Article
- Gabapentin Disrupts Binding of Perlecan to the αBiomolecules · 2022Article
- Pathways Controlling Formation and Maintenance of the Osteocyte Dendrite Network.Current osteoporosis reports · 2022Review
- Osteocytes Serve as a Reservoir for Intracellular PersistingFrontiers in microbiology · 2022Article
- Estrogen depletion on In vivo osteocyte calcium signaling responses to mechanical loading.Bone · 2021Article
- Regulation of the integrin αVβ3- actin filaments axis in early osteogenesis of human fibroblasts under cyclic tensile stress.Stem cell research & therapy · 2021Article
- Review
- A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction.Nature communications · 2020Article
- Molecular mechanosensors in osteocytes.Bone research · 2020Review
- Microtubules tune mechanotransduction through NOX2 and TRPV4 to decrease sclerostin abundance in osteocytes.Science signaling · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Osteocytes are bone cells that form cellular networks that sense mechanical loads distributed throughout the bone tissue. Interstitial fluid flow in the lacunar canalicular system produces focal strains at localized attachment sites around the osteocyte cell process. These regions of periodic attachment between the osteocyte cell membrane and its canalicular wall are sites where pN-level fluid-flow induced forces are generated in vivo. In this study, we show that focally applied forces of this magnitude using a newly developed Stokesian fluid stimulus probe initiate rapid and transient intercellular electrical signals in vitro. Our experiments demonstrate both direct gap junction coupling and extracellular purinergic P2 receptor signaling between MLO-Y4 cells in a connected bone cell network. Intercellular signaling was initiated by pN-level forces applied at integrin attachment sites along both appositional and distal unapposed cell processes, but not initiated at their cell bodies with equivalent forces. Electrical coupling was evident in 58% of all cell pairs tested with appositional connections; coupling strength increased with the increasing number of junctional connections. Apyrase, a nucleotide-degrading enzyme, suppressed and abolished force-induced effector responses, indicating a contribution from ATP released by the stimulated cell. This work extends the understanding of how osteocytes modulate their microenvironment in response to mechanical signals and highlights mechanisms of intercellular relay of mechanoresponsive signals in the bone network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.